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Updated: Jun 25, 2026

Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors
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Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors

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通过DNA纳米装置检测通道的有机体特异性活性

Palapuravan Anees1,2,3, Anand Saminathan1,2, Ezekiel R Rozmus4

  • 1Department of Chemistry, The University of Chicago, Chicago, IL, USA.

Nature biotechnology
|September 21, 2023
PubMed
概括

通常在细胞表面发现的 (K+) 通道在有机体内是活跃的. 新的pHlicKer技术揭示了这些通道.

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Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...