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通过Pseudomonas plecoglossicide MG2进行高效的马六合绿色生物降解:过程优化,生物反应器中的应用和降解途径

Magda A El-Bendary¹, Mariam E Fawzy², Mohamed Abdelraof³

¹Microbial Chemistry Department, Biotechnology Research Institute, National Research Centre, 33 Bohouth St., Dokki, Giza, Egypt. tasnim41@yahoo.com.

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概括

这项研究表明,Pseudomonas plecoglossicide MG2有效降解了马六甲绿 (MG) 染料,达到99%以上的降解,并消除了废水中的95.95%的COD. 由此产生的副产品是无毒的,为染料废水处理提供了可持续的解决方案.

科学领域:

环境微生物学环境微生物学
生物技术是生物技术.
废水处理废水处理

背景情况:

合成染料因其持久性和毒性而带来环境挑战.
微生物降解为染料排毒提供了一种绿色,成本效益和环保的方法.
马拉绿 (MG) 是一种被广泛使用的染料,具有已知的细胞毒性作用.

研究的目的:

用一种新分离的细菌,Pseudomonas plecoglossicide MG2来研究马拉绿 (MG) 的脱色和降解.
为了优化MG生物降解的条件.
分析降解产物并评估它们的细胞毒性.

主要方法:

隔离和识别Pseudomonas plecoglossicide MG2的MG2. 这种病毒的分离和识别.
使用UV-Vis光谱光度计优化MG生物降解.
通过液态染色学-质谱学 (LC-MS) 和富里埃变换红外光谱学 (FTIR) 分析降解产品.
对人类正常视网膜细胞系的细胞毒性评估.

主要成果:

在静态有氧条件下,MG脱色的最佳条件 (90-93%) 是pH值为6-7,注射剂大小为4-6%,温度为30-35°C.
在生物反应器中,Pseudomonas plecoglossicide MG2实现了99%的MG降解和95.95%的COD去除,性能优于活性污泥.
LC-MS和FTIR确定了12种降解中间体,并提出了两种降解途径. 生物降解产品与MG本身不同,对人类视网膜细胞没有毒性.

结论:

Pseudomonas plecoglossicide MG2 是一种高效的微生物,用于马拉绿色染料的生物降解.
这种细菌为处理含有染料的废水提供了一种可持续,经济有效和环保的生物技术解决方案.
降解产品的无毒性强调了这种微生物处理方法的环境安全性.

关键词:

生物反应器是一种生物反应器.细胞毒性细胞毒性降解降解是一种降解.降解途径的降解途径马六合绿色的绿色是马六合绿色的优化优化优化优化伪omonas 是一个类型的.光谱学表征的表征

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