尼斯和诺奇3协同调节肺内皮细胞中的血管生成,原生成和内皮-介质细胞过渡
Wakako Daido1, Taku Nakashima2, Takeshi Masuda1
1Department of Molecular and Internal Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-Ku, Hiroshima, 734-8551, Japan.
Cell communication and signaling : CCS
|September 22, 2023
概括
肺内表达细胞是一种独特的内皮细胞类型,通过Notch3.3调节血管生成,原生成和内皮-介质酶过渡 (EndoMT). 尼斯在肺纤维化期间不会增加.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 肺部研究 肺部研究
背景情况:
- 尼斯是一种中间丝蛋白,涉及到伤口愈合,血管新生,内皮-介质细胞过渡 (EndoMT) 和纤维化.
- 由于研究结果相互矛盾,肺内斯表达细胞的特定作用尚不清楚.
研究的目的:
- 描述肺内表达尼斯细胞的性质和功能.
- 为了研究内斯在肺血管生成,EndoMT和纤维化中的参与.
主要方法:
- 使用了体外研究与内皮细胞系MS1和体内实验与巢素-GFP小鼠用于细胞检测的实验.
- 采用了nestin基因沉默 (siRNA) 并分析了Nes,Col1a1和Notch3.3的基因表达.
- 研究的细胞表面标记物包括CD45,CD31,PDGFRβ和VEGFR2.
主要成果:
- 肺内斯表达细胞是一种CD31+内皮细胞亚群,与内斯不表达的内皮细胞不同.
- 尼斯的表达与血管生成和Notch3调节具有积极的相关性,影响着原蛋白的产生和EndoMT.
- 与之前的观点相反,在肺纤维化期间,内斯表达和内斯表达细胞的表达并没有增加.
结论:
- 肺体表达细胞代表了一个独特的内皮细胞系,与Notch3合作调节血管生成,原蛋白生产和EndoMT.
- 尼斯在肺血管生成和纤维化过程中起到以前未知的作用.
- 这项研究阐明了表达内斯的细胞的功能及其在肺病理生理学中的参与.
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