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基于化学测量方法,对来自不同地区的Physochlainae Radix进行定性鉴定和定量比较

Zhenguo Lv¹, Guangzhe Yao¹, Minglei Ge¹

¹State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.

Journal of separation science

|September 22, 2023

概括

用一个全面的战略来评估Physochlainae Radix (PR) 的质量. 超高性能液体染色学与质谱学 (UHPLC-MS) 和化学测量相结合,确定了区分PR来源和质量的关键化合物.

科学领域:

药理学是指药理学,即药理学是指药理学.
分析化学分析化学
化学测量化学测量化学测量

背景情况:

草根 (Physochlainae Radix,PR) 是一种重要的草药,用于治疗咳和喘.
标准化质量评估对于确保PR的有效性和安全性至关重要.

研究的目的:

建立一个全面的策略来评估来自不同来源的公关质量.
确定主要的化学标记物,以区分PR生产区和产品质量.

主要方法:

超高性能液态染色学与四极飞行时间联质谱学 (UHPLC-Q-TOF-MS/MS) 结合,用于化学分析.
超高性能液态色谱与三重四极质谱仪 (UHPLC-QqQ-MS/MS) 结合用于定量分析.
多变量统计分析用于PR样本的歧视性分析.

主要成果:

在PR中确定了58种化学成分.
开发了一个快速的UHPLC-QqQ-MS/MS方法来量化12种化合物.
根据化学配置文件,将30个PR批量分为三个类别.
斯科帕龙,protocatechuic酸,热带酸和scopolin被确定为质量差异化的重要标记.

结论:

经过LC-MS和多变量统计分析的综合方法为PR质量评估提供了一个强大的方法.
该战略有效地区分了初级和非初级生产区域,并评估了产品质量.
这些发现有助于标准化和质量控制 Physochlainae Radix.

关键词:

物理线 (Physochlainae Radix) 是一个线.化学测量方法化学测量方法草药是一种草药.质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量评价质量评估质量评价

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