针对威尔姆斯瘤的WT1-反应性T细胞的细胞毒性:对抗原特异性采用免疫疗法的影响
Seyed Mostafa Monzavi1,2,3, Amir Ali Hamidieh4, Mohammad Vasei5
1Department of Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
BioImpacts : BI
|September 22, 2023
概括
研究人员成功地从威尔姆斯瘤患者中分离了WT1反应性T细胞,证明了它们对威尔姆斯瘤细胞的强大抗瘤活性. 这些T细胞显示出作为WT1表达威尔姆斯瘤的采用性免疫治疗的前景.
科学领域:
- 免疫学 免疫学 免疫学
- 在瘤学瘤学.
- 细胞疗法细胞疗法
背景情况:
- 威尔姆斯瘤是一种儿童癌症,其特征是WT1表达.
- 特定于WT1的T细胞可以消除WT1表达的瘤细胞.
- 研究WT1反应性T细胞的隔离和有效性对于新型癌症疗法至关重要.
研究的目的:
- 确定从健康捐赠者和威尔姆斯瘤患者中分离WT1反应性T细胞的可行性.
- 评估这些孤立的T细胞对威尔姆斯瘤细胞 (WiTu细胞) 的细胞毒性活性.
主要方法:
- 用WT1刺激了周围血液单核细胞 (PBMC).
- 使用基于干扰素γ捕获的免疫磁分离 (IMS) 丰富了WT1-反应性T细胞.
- 用乳酸脱酶释放试验评估细胞毒性,并与标准化疗进行比较.
主要成果:
- 与健康捐赠者相比,从威尔姆斯瘤患者中分离出WT1反应性T细胞的比例更高.
- 隔离的WT1反应性T细胞对WT1+WiTu细胞表现出显著的特异性溶解 (>50%).
- 与标准化疗相比,WT1反应性T细胞表现出剂量依赖的抗瘤活性和更高的细胞毒性.
结论:
- 可以有效地从威尔姆斯瘤患者的PBMC中丰富WT1反应性T细胞.
- 活体生成的WT1反应性T细胞代表了WT1+威尔姆斯瘤的潜在采用性免疫疗法.
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