不同的血小板激活通过与不同SARS-CoV-2变种的尖端蛋白相互作用
Ziv Sevilya1, Alona Kuzmina2, Michal Cipok3
1Cardiology Department, Assuta Ashdod Medical Center, Ashdod, Israel. zivse@assuta.co.il.
Journal of thrombosis and thrombolysis
|September 22, 2023
概括
COVID-19 变种不同激活血小板,影响血栓形成的风险. 德尔塔变种显示出血小板激活率最高,与临床严重程度相关.
科学领域:
- * 血液学 血液学
- * 病毒学 病毒学
- * 免疫学 免疫学
背景情况:
- *COVID-19与增加的血栓性并发症和死亡率有关.
- *在COVID-19患者中,血小板激活和反应性增加.
- *SARS-CoV-2可能与血小板直接相互作用并激活血小板.
研究的目的:
- * 为了比较不同SARS-CoV-2尖端变异对血小板激活的影响.
- * 调查变体诱导的血小板激活与临床结果之间的关系.
主要方法:
- * 具有SARS-CoV-2尖端变体 (武汉-胡,三角洲,阿尔法,欧米克朗BA.1,欧米克朗BA.2) 伪型的工程化lentiviral颗粒.
- *将伪型颗粒与健康个体的富血小板血进行化.
- *通过流细胞计量测量血小板激活标记物 (CD62P和激活的αIIbβ3).
主要成果:
- * 野生型武汉-胡尖端蛋白增加了CD62P,并分别激活了3.5和3.3倍的αIIbβ3.
- * 德尔塔变种诱导的血小板激活显著增加 (CD62P的6.6倍,αIIbβ3的5.0倍).
- *欧米克朗BA.1和阿尔法变种显示了最低的激活水平 (1.6-1.8倍).
- * Omicron BA.2诱导的激活与武汉-胡菌株相当 (CD62P的2.8倍,αIIbβ3的2.1倍).
结论:
- *SARS-CoV-2尖端变种表现出明显的血小板激活能力.
- * 在测试的变种中,Delta变种表现出最强的血小板激活能力.
- *观察到的血小板激活的差异与与每个变体相关的临床严重程度和死亡率相关.
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