在Aspergillus niger中,通过控制降水量来改善生物溶解的监管系统方法
Ali Naderi1, Farzane Vakilchap1, Ehsan Motamedian1
1Biotechnology Group, Chemical Engineering Department, Tarbiat Modares University, Tehran, Iran.
Applied microbiology and biotechnology
|September 22, 2023
概括
研究人员设计了Aspergillus niger的新陈代谢,以偏好酸而不是氧酸,以提高电子废物生物溶解. 这种方法显著改善了从印刷电路板中回收的铜和.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 环境科学 环境科学
背景情况:
- 电子垃圾的真菌生物溶解面临的挑战是氧酸的有毒金属沉.
- 酸为溶解和等金属提供了卓越的化特性.
- 目前的氧化酸减少方法不经济,需要高碳源度.
研究的目的:
- 开发一种系统代谢工程方法,将真菌代谢从氧酸转向酸生产.
- 提高废弃电信印刷电路板 (TPCB) 中的铜和的生物炼效率.
- 确定有效抑制氧酸生产的抑制剂,促进酸合成.
主要方法:
- 利用Aspergillus niger的基因组规模代谢模型来预测流量变化和确定关键的调节反应.
- 雇佣了BRENDA监管机构和一个分子对接方法来定义监管策略.
- 选了真菌培养中的配体,并为分子对接而建模了氧化酸化酶.
- 应用了甲胺,谷氨和化等抑制剂来控制细胞代谢.
主要成果:
- 成功地将代谢流量从酸转换为酸生产,达到8100 ppm的酸.
- 甲胺被确定为氧酸生产的有效抑制剂.
- 间接生物洗显示没有沉,并且铜和的回收率分别增加到61%和100%.
- 铜的生物化效率提高了21%,的效率提高了76%.
结论:
- 建立了一种新的调节系统程序,用于控制黑色阿斯伯菌的细胞代谢.
- 设计的代谢途径显著提高了从TPCB中提取有价值金属的生物化.
- 这种方法提供了一种更经济,更有效的解决方案,通过真菌生物洗来回收电子废物.
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