乌尔夫尼奥丁D-N,具有抗寨卡病毒活性的结构多样化的迪特尔类,从Orthosiphon wulfenioides中分离出来
Wen-Chao Tu1,2, Yong-Xiang Huang3, Bo Li2
1Key Laboratory of Functional Molecular Engineering of Guangdong Province, School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, 510640, People's Republic of China.
Journal of natural products
|September 22, 2023
概括
研究人员从Orthosiphon wulfenioides中分离出11种迪特尔,发现化合物3和5通过抑制病毒包膜蛋白表达,对寨卡病毒 (ZIKV) 产生活性.
科学领域:
- 自然产品化学 自然产品化学
- 病毒学 病毒学
- 药用化学 医学化学
背景情况:
- 奥尔托西 (Orthosiphon wulfenioides) 是一种植物来源的多种化学化合物.
- 寨卡病毒 (ZIKV) 构成了严重的全球健康威胁,需要新的治疗策略.
研究的目的:
- 从Orthosiphon wulfenioides中分离和特征化diterpenoids. 为了分离和特征化diterpenoids.
- 评估隔离的化合物对ZIKV的抗病毒活性.
主要方法:
- 通过光谱方法,X射线衍射和电子圆形二极化,对11个二极管的隔离和结构阐明.
- 对ZIKV的抗病毒活性查,包括对Vero细胞的细胞毒性测定.
- 使用西斑和免疫光来评估ZIKV包裹 (E) 蛋白质表达的作用研究的机制.
主要成果:
- 确定了11种双类动物,D-N (1-11) 类 wulfenioidins,其中包括一种新的碳骨架.
- 化合物3和5显示出强大的ZIKV抑制,EC50值分别为8.07和8.50μM.
- 化合物3和5在100μM时没有显著的细胞毒性,并且被发现可以抑制ZIKV E蛋白表达.
结论:
- 奥尔托西 (Orthosiphon wulfenioides) 是一种有价值的生物活性二甲的来源.
- 化合物3和5代表了ZIKV治疗药物的有希望的线索,因为它们的疗效和向病毒复制的作用机制.
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