甲基因组测序揭示了非细分性白风患者的肠道微生物组成和基因功能的改变
Mei Luan1, Mengtian Niu1, Pengju Yang1
1Department of Dermatology, the Frist Affiliated Hospital of Xi'an Jiaotong University, 277 West Yanta Road, 710061, Xi'an, Shaanxi, People's Republic of China.
BMC microbiology
|September 22, 2023
概括
与健康个体相比,白病患者的肠道微生物群多样性和组成发生了变化. 确定了特定的微生物标记物和代谢途径,为早期诊断和向治疗提供了潜力.
科学领域:
- 微生物组研究的研究.
- 皮肤病学 皮肤病学
- 转基因组学是指转基因组学.
背景情况:
- Vitiligo 越来越多地与肠道微生物群失调有关.
- 了解这些肠道微生物变化对于非细分性白风 (NSV) 至关重要.
研究的目的:
- 系统地描述NSV患者的肠道微生物组成,遗传功能和代谢特征.
- 识别独特的微生物特征,使NSV患者与健康对照者 (HCs) 有区别.
主要方法:
- 在25名NSV患者和25名HC患者身上进行了元基因组测序和生物信息分析.
- 分析了肠道微生物群的多样性,组成,基因功能 (KEGG) 和代谢模块 (MetaCyc).
- 线性差异分析 (LDA) 效果大小 (LEfSe) 确定了关键的微生物标记物.
主要成果:
- 在NSV患者中,肠道微生物群的α多样性显著减少.
- 关键的物种差异包括降低了Staphylococcus热友菌和增加了Bacteroides fragilis.
- 在NSV患者中发现了明显的微生物标记物 (例如,Bacteroides fragilis) 和改变的代谢途径 (囊和银河糖降解).
- 一个由28个特征组成的小组有效地区分了NSV患者和HC患者.
结论:
- 白病患者的肠道微生物概况和遗传功能与HC不同.
- 已识别的微生物标记物和途径代表了早期诊断和白风治疗干预的潜在目标.
相关概念视频
01:29Modern Molecular Taxonomy
43
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
43
01:33Pleiotropy
40.6K
Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...
40.6K