在Panicum hallii中基因表达分歧的进化分析:利用相互移植探索构成性和塑性反应
Govinal Badiger Bhaskara1, Taslima Haque1, Jason E Bonnette1
1Department of Integrative Biology, The University of Texas at Austin, Austin, TX, USA.
Molecular biology and evolution
|September 22, 2023
概括
在Panicum hallii中,局部适应是由cis调节差异驱动的,导致显著的基因表达变化. 这项研究强调了cis多态性作为基因表达和局部适应进化的关键因素.
科学领域:
- 进化生物学 进化生物学
- 基因组学就是基因组学.
- 植物科学 植物科学
背景情况:
- 基因表达进化对于局部适应和生态物种化至关重要.
- 表达的分歧源于 cis-regulatory 和 trans-regulatory 的因素.
研究的目的:
- 调查其范围内的Panicum hallii的表达和序列分歧.
- 为了确定基因和调节机制驱动本地适应.
主要方法:
- 相互移植实验,使用大量的Panicum hallii加入样本.
- 修改FST-QST异常值方法 (QPC分析) 用于检测局部适应.
- 在促进体和编码区域的序列分歧的种群遗传分析.
主要成果:
- 观察到广泛的基因型和移植部位驱动器的表达分歧.
- 确定了514个具有构成性表达分歧的基因 (QPC异常值).
- 基因表达异的丰富与cis-regulatory和cis-environment相互作用;没有与转变因子的关联.
结论:
- 基因调节差异是Panicum hallii中基因表达差异的主要驱动因素.
- 促进体和构成表达异常值的编码序列的序列分歧增加.
- 对这些异常基因蛋白质的积极选择的证据有限.
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