与衰老相关的线粒体功能障碍与良性前列腺增生症中的纤维化有关
Alexis E Adrian1, Teresa T Liu1, Laura E Pascal2,3
1Department of Urology, George M. O'Brien Center of Research Excellence, University of Wisconsin-Madison, Madison, Wisconsin, USA.
概括
线粒体功能障碍有助于良性前列腺激增症 (BPH) 和衰老中的尿道问题. 油酸 (OA) 可能通过绕过复杂I抑制来改善BPH症状,提供一种新的治疗途径.
科学领域:
- 泌尿器科 泌尿器科 泌尿器科 泌尿器科
- 老年学是一门学科.
- 线粒体生物学 线粒体生物学
背景情况:
- 衰老是下泌尿道症状/良性前列腺增生 (LUTS/BPH) 的主要危险因素.
- 由于纤维化和炎症等病理特征,对LUTS/BPH的药物治疗可能会失败.
- 线粒体功能障碍在BPH病理中的作用,这是衰老的标志,在很大程度上是未知的.
研究的目的:
- 研究线粒体功能障碍对BPH病理特征的影响.
- 探索油酸 (OA) 作为LUTS/BPH的治疗剂的潜力.
主要方法:
- 对人类前列腺组织中线粒体蛋白质的基因阵列数据和免疫组织化学的分析.
- 在体外研究中,使用qPCR,免疫细胞化学和海马测定检查前列腺细胞的复杂I抑制效应.
- 在体内研究中,老年小鼠接受了OA治疗,以评估尿功能和前列腺NDUFS3表达.
主要成果:
- BPH组织显示线粒体功能和纤维化基因发生变化,关键线粒体蛋白质减少 (例如,NDUFS3).
- 细胞中的复合I抑制减少了呼吸,改变了NDUFS3和增加了原沉积;OA治疗改善了这些效应.
- 接受OA治疗的老年小鼠表现出改善的排泄功能和增加的前列腺NDUFS3水平.
结论:
- 复杂I功能障碍可能导致老年小鼠的纤维化和尿路功能障碍.
- 油酸 (OA) 通过部分绕过复杂I抑制,显示出作为LUTS/BPH的线粒体调节器的潜力.
- 这项研究确定了一种用于管理LUTS/BPH的新型细胞标.
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