相关实验视频
Updated: Jul 16, 2025
10:43
Modeling Oral-Esophageal Squamous Cell Carcinoma in 3D Organoids
Published on: December 23, 2022
3.4K
STAT3-EphA7轴有助于食道状细胞癌的进展
Li Wang1, Qiao-Feng Zhao2,3, Bing-Bing Yang2,3,4
1Department of Obstetrics and Gynecology, Center of Genetics and Prenatal Diagnosis, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, P. R. China.
Acta oncologica (Stockholm, Sweden)
|September 22, 2023
概括
信号转换器和转录3激活器 (STAT3) 信号激活了食道状细胞癌 (ESCC) 中的EphA7表达. 这种STAT3-EphA7轴促进ESCC细胞增殖和瘤生长,突出了潜在的治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 癌症研究 癌症研究
背景情况:
- 之前的研究发现,在食道状细胞癌 (ESCC) 中,EphaA7的升高调节与激活STAT3.3的异种移植.
- 在ESCC中,连接EphA7和STAT3的确切机制尚不清楚.
研究的目的:
- 调查ESCC中EphA7和STAT3之间的关联.
- 通过STAT3.3阐明EphA7的调节机制.
- 评估EphA7在ESCC进展中的功能作用.
主要方法:
- 西部涂抹,免疫光,ChIP测定和qRT-PCR被用于评估EphA7和STAT3的相互作用.
- 路西法酶测定和截断突变分析确定了由STAT3.3调节的EphA7促销器区域.
- 细胞增殖试验 (CCK-8,殖民地形成) 和异种移植模型评估了EphA7对ESCC生长的影响.
- 通过RNA测序分析了在EphA7调制后的信号通路变化.
主要成果:
- 在ESCC细胞系和组织中,EphA7表达与STAT3激活正相关.
- STAT3直接调节了EphA7的转录,其中 -2000∼-1500区域被确定为关键促进体.
- 抑制STAT3降低了EphA7蛋白和转录,而IL-6诱导的STAT3激活则上调了EphA7.
- 过度表达EphA7增强了ESCC细胞增殖和瘤生长,而抑制EphA7则抑制了这些效应.
- EphA7抑制了被废除的STAT3激活驱动的ESCC细胞增殖.
- 在EphA7下调后,RNA-seq显示了与瘤相关的信号通路的显著变化.
结论:
- 激活的STAT3在ESCC中通过转录升高调节EphA7的表达.
- STAT3-EphA7信号轴在促进ESCC发展和进步方面发挥着至关重要的作用.
- 针对STAT3-EphA7通路可能为ESCC提供一种新的治疗策略.
相关概念视频
02:07Tumor Progression
6.4K
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
6.4K
01:21Barrett Esophagus-I: Introduction
116
Barrett's esophagus is a medical condition where the esophageal mucosa is significantly damaged by stomach acid or other digestive fluids, often due to long-term exposure associated with gastroesophageal reflux disease (GERD). In GERD, a weakened or abnormally relaxed lower esophageal sphincter allows stomach acid to flow persistently into the esophagus.
This constant acid exposure transforms the esophagus's pink mucosal lining (stratified squamous epithelium) into a type of lining more...
This constant acid exposure transforms the esophagus's pink mucosal lining (stratified squamous epithelium) into a type of lining more...
116
01:22Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
2.2K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.2K
01:24Esophagus
980
The esophagus, a muscular conduit linking the pharynx and stomach, measures roughly 10 inches (25.4 cm) and sits behind the trachea. It remains collapsed when not swallowing. The esophagus follows a predominantly straight path through the thoracic mediastinum and enters the abdominal cavity through a diaphragmatic opening known as the esophageal hiatus.
The movement of edibles from the pharynx into the esophagus is facilitated by the upper esophageal sphincter, which is formed primarily by the...
The movement of edibles from the pharynx into the esophagus is facilitated by the upper esophageal sphincter, which is formed primarily by the...
980