perfluorooctanoic 酸诱导转录的变化在第二个三个月的人类细胞因子细胞
Hao Chen1, Mirhan Kapidzic1, Danielle Gantar1
1Center for Reproductive Sciences, Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco (UCSF), San Francisco, California 94143, USA.
Toxicological sciences : an official journal of the Society of Toxicology
|September 22, 2023
概括
像PFOA一样的Per-和多醇基物质 (PFAS) 损害人体细胞原体,影响细胞活力和基因表达. 这表明可能存在胎盘功能障碍和产前PFAS暴露的疾病.
科学领域:
- 环境健康 环境健康
- 毒理学 毒理学 毒理学
- 生殖生物学 生殖生物学
背景情况:
- 聚和 perfluoroalkylated 物质 (PFAS) 是在全球范围内在人类组织中发现的持久性工业化学品.
- 产前暴露于PFAS,包括 perfluorooctanoic acid (PFOA),与不良妊娠结果有关,但潜在的机制尚不清楚.
- 新兴研究表明,PFAS可能会准胎盘并损害 trofhoblast 功能.
研究的目的:
- 为了研究PFOA对人类细胞质细胞 (CTBs) 中的细胞毒性和基因表达的影响.
- 在胎盘细胞中识别受PFOA影响的特定分子通路.
主要方法:
- 主要人体第二季度CTB被培养并暴露在不同度的PFOA中.
- 细胞活力在24小时后被评估.
- 进行了转录组分析,以确定基因表达变化.
- 使用in silico分析来预测调节途径.
主要成果:
- PFOA显著降低了CTB的活力,并以度依赖的方式诱导细胞死亡.
- 亚细胞毒性PFOA度改变了数百个基因的表达.
- 受影响的基因包括那些参与脂质代谢和先天免疫反应的基因 (例如,CRH,IFIT1,TNFSF10).
- 在 silico 分析中涉及过氧体增殖器激活受体 (PPAR) 途径.
结论:
- PFOA直接影响人类细胞质细胞活力,并改变关键的基因表达途径.
- 这些发现表明PFOA可能导致胎盘功能障碍和不良妊娠结果的机制.
- 需要进一步研究PFOA对胎盘健康的影响.
更多相关视频
08:26Transcriptional Analysis by Nascent RNA FISH of In Vivo Trophoblast Giant Cells or In Vitro Short-term Cultures of Ectoplacental Cone Explants
Published on: August 31, 2016
7.9K
09:57In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines
Published on: March 5, 2019
10.2K
相关概念视频
01:07Teratogenicity
2.5K
The ability of a drug to produce structural deformations and functional abnormalities in the developing embryo or the fetus is called teratogenicity, and the drug producing this effect is known as a teratogen. Teratogenic effects include stillbirth, miscarriage, intrauterine growth restriction, and neurocognitive delay. A teratogen may affect the embryo at different stages of development, which is important in determining the type and extent of the damage. During blastocyst formation, the early...
2.5K
01:15Transcytosis of IgG
2.8K
Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis.
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
2.8K