Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.
Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Type I unconventional protein secretion of the SARS-CoV-2 nucleocapsid protein promotes inflammatory cytokine release.

Cell reports·2026
Same author

Toward quantum sensing of electron beams using solid-state spins.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

A 3D-Printed Scaffolded Hydrogel Microneedle Array Biosensor for Real-Time, Continuous Monitoring.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Continuous monitoring of blood-interstitial fluid intercompartmental molecular kinetics in freely moving animals.

Science advances·2026
Same author

Engineered 3D-Lattice Microneedle Array Patches for Enhanced Nanovaccine Delivery to Dendritic Cells in Cancer Immunotherapy.

ACS nano·2026
Same author

Early Life Microplastic Exposure Impairs Mouse Incisor Enamel Formation.

Calcified tissue international·2026

相关实验视频

Updated: Jun 14, 2026

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications
14:43

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Published on: September 23, 2013

10.9K

一个基于抗体的分子开关,用于连续的小分子生物传感.

Ian A P Thompson1, Jason Saunders1, Liwei Zheng2

  • 1Department of Electrical Engineering, Stanford University, Stanford, CA 94305, USA.

Science advances
|September 22, 2023
PubMed
概括

我们开发了用于实时,无样本的小分子生物传感的抗体开关. 这种模块化设计使生物样本中的皮质醇等生物标志物的持续光学检测成为可能.

更多相关视频

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

15.2K
Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
15:27

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Published on: April 17, 2017

20.9K

相关实验视频

Last Updated: Jun 14, 2026

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications
14:43

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Published on: September 23, 2013

10.9K
A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

15.2K
Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
15:27

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Published on: April 17, 2017

20.9K

科学领域:

  • 生物医学工程 生物医学工程
  • 分子生物学分子生物学
  • 分析化学 分析化学

背景情况:

  • 对小分子生物标志物的持续监测对于诊断至关重要.
  • 现有的生物传感方法通常需要样本准备,缺乏实时检测能力.

研究的目的:

  • 开发一种可通用的方法来创建基于抗体的生物传感器,能够连续,实时和无需样本准备的检测小分子生物标志物.
  • 为光学生物传感应用设计"抗体开关".

主要方法:

  • 通过DNA脚手架将它们与分子竞争对手联系起来,将抗体工程化为响应目标的"抗体开关".
  • 目标分析物的竞争性结合诱导了支架切换和光信号变化.
  • 该系统被证明可以在未稀释的血中感知迪戈西基宁和皮质醇.
  • 通过改变对皮质醇检测的分子竞争对手来实现灵敏度调制.
  • 抗体开关与光纤传感器集成,用于持续监控.

主要成果:

  • 在未稀释血中实现了快速,无需样本制备的迪戈西基宁和皮质醇检测.
  • 皮质醇开关在广泛的度范围 (3.3 nM至3.3 mM) 中显示出可调节的灵敏度.
  • 在缓冲和血液中持续感知皮质醇,时间分辨率低于5分钟,成功证明了这一点.

结论:

  • 开发的抗体切换平台为连续生物传感提供了模块化和多功能的方法.
  • 这项技术有可能为广泛的小分子生物标志物开发实时生物传感器.
  • 该方法消除了对样本准备的需要,简化了生物传感程序.