鼠类上腺皮层和松果腺在反转光暗周期中的变化:生物化学,组织学和免疫组织化学研究
Heba A Shawky1, Sara Mohamed Naguib Abdel Hafez2, Nabil Abdel Kader Hasan1
1Anatomy and Embryology Department, Faculty of Medicine, Minia University, Minia Governorate, Minia City, Cairo-Aswan Agricultural Road, El-Minia 61511, Egypt.
概括
破坏自然的光暗循环会对上腺和松果腺产生负面影响. 这项研究表明,反向光照会在老鼠中引起显著的应激反应和组织损伤.
科学领域:
- 内分泌学 在内分泌学.
- 时间生物学 时间生物学
- 组织病理学 组织病理学
背景情况:
- 睡眠不足很普遍,并与增加的压力有关.
- 上腺和上腺是应激反应和睡眠调节的关键.
- 扰乱昼夜节律可能会影响内分泌功能.
研究的目的:
- 为了研究反转光暗周期对上腺皮质和松果腺的组织学影响.
- 评估由昼夜干扰引起的生化和免疫组织化学变化.
主要方法:
- 成年雄性白色老鼠被暴露在12小时的光-12小时的黑暗周期 (对照) 或反转周期4周.
- 上腺经历了生物化学,组织学和免疫组织化学分析.
- 松果腺的变化也进行了组织学检查.
主要成果:
- 反转光暗循环组显示了血皮质的升高,氧化应激标记物 (甲) 和炎症性细胞因子 (TNF-α,IL-1β).
- 在逆循环组中观察到抗氧化酶 (catalase,SOD) 的降低.
- 组织学检查显示,上腺皮层受损,松果腺结构损失,iNOS,Bax和Bcl-2的表达变化.
结论:
- 亚慢性反转光暗周期显著影响上腺皮质和松果腺的结构和功能.
- 循环节律的扰乱会诱导一种亲炎和亲氧化状态.
- 这些发现凸显了改变光线暴露对内分泌健康的有害影响.
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