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Updated: Jul 16, 2025

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Following Cell-fate in E. coli After Infection by Phage Lambda
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通过条件表达一个prophage编码的holin产生Lactobacillus plantarum幽灵
Pinpunya Riangrungroj1, Wonnop Visessanguan1, Ubolsree Leartsakulpanich1
1National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Klong Luang, Pathum Thani 12120, Thailand.
FEMS microbiology letters
|September 22, 2023
概括
研究人员使用Lactobacillus plantarum,一种食品级细菌,开发出更安全的细菌幽灵 (BGs). 这种新型的holin诱导的L. plantarum BG平台为生物分子传递应用提供了更安全的替代方案.
科学领域:
- 生物技术是生物技术.
- 微生物学 微生物学
- 分子生物学分子生物学
背景情况:
- 细菌幽灵 (BGs) 是用于生物医学应用的不可活性的细菌细胞外.
- 安全问题源于BG生产过程中致病细菌的不完全失活.
- 为了可靠的BG制造,需要更安全的细菌来源.
研究的目的:
- 开发一种使用食品级细菌的更安全的细菌幽灵 (BG) 生产系统.
- 为了研究一个prophage编码的holin (LpHo) 对于产生L. plantarum BGs.的有效性.
- 建立一个新的平台,用于生物分子的交付.
主要方法:
- 使用费洛蒙可诱导的PSIP系统对Lactobacillus plantarum prophage编码的holin (LpHo) 的条件表达.
- 将LpHo定位到细胞膜.
- 在LpHo诱导时评估细胞活力,膜完整性和核酸释放.
主要成果:
- 在L. plantarum中,LpHo的表达导致显著的生长迟缓和细胞活力下降.
- 扫描电子显微镜证实了膜孔的形成.
- 流细胞计表明了膜脱极化和核酸释放,证实了幽灵的形成.
结论:
- 霍林诱导的L. plantarum BG平台为从致病性细菌中获得的BG提供了更安全的替代方案.
- 该系统通过受控的膜通透来证明了高效的细菌幽灵形成.
- L. plantarum BG平台具有安全有效的生物分子传递的潜力.
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