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在细菌性脑膜炎中,用于声动力消除耐抗生素细菌的磁性向的外膜囊泡

Rui Shi¹, Rui Lv², Ziliang Dong³

¹Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Soochow University, 199 Ren'ai Rd, Suzhou, 215123, Jiangsu, PR China; University of Groningen and University Medical Center Groningen, Department of Biomedical Engineering, Antonius Deusinglaan 1, 9713 AV, Groningen, the Netherlands.

|September 22, 2023

概括

磁性纳米粒子和外膜囊泡通过血脑屏障传递抗生素,用于治疗细菌性脑膜炎. 这种新的方法提高了生存率,并防止了老鼠模型中的复发.

科学领域:

纳米技术纳米技术
生物医学工程生物医学工程
传染性疾病传染性疾病

背景情况:

血脑屏障限制了治疗细菌性脑膜炎的抗生素输送.
外膜囊泡 (OMVs) 是仿生纳米载体,但可以引起炎症.
氧化铁磁纳米粒子诱导E. coli中磁性OMVs的分泌.

研究的目的:

开发非炎症性磁性OMVs用于跨越血脑屏障的向药物输送.
为了研究 ceftriaxone 和 sonodynamic 治疗细菌性脑膜炎的协同效应.
在脑膜炎的小鼠模型中评估磁性OMV的疗效.

主要方法:

通过使用CRISPR/Cas9.9来制造非炎症性磁性OMV的工程大肠杆菌.
装载有塞夫特里亚克松 (CRO) 和美索-四-4-氧) 孔氨酸 (TCPP) 的OMV.
在小鼠脑膜炎模型中利用磁性准和超声波进行药物输送和声动疗法.

主要成果:

非炎性磁性OMVs成功地准备并装载了CRO和TCPP.
装有CRO和TCPP的OMV在体外显示出协同作用的抗菌活性.
磁性准和超声波应用显著改善了小鼠的生存率和临床评分.
在治疗后至少两周内,没有观察到感染的复发.

结论:

非炎症性磁性OMV为克服血脑屏障提供了一个有希望的平台.
这种方法使细菌脑膜炎的有效声动力学治疗成为可能,包括耐抗生素菌株.
磁性准和超声波激活的药物输送系统显示出治疗中枢神经系统感染的潜力.

关键词:

抗生素抗生素是一种抗生素.血液-大脑屏障是什么?膜囊泡是一种膜囊泡.脑膜炎是一种脑膜炎.复发情况复发情况声动力学疗法声动力学疗法

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