一个基于ACHE的电化学生物传感器用于有机酸盐农药,使用印碳电极上的改性CuNWs/rGO纳米复合材料
Jakkrit Suwannachat1, Apichart Saenchoopa1, Wonn Shweyi Thet Tun2
1Materials Chemistry Research Center, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.
Food chemistry
|September 22, 2023
概括
一种新的酶生物传感器通过测量乙胆酶 (AChE) 抑制来检测有机酸盐 (OPs). 该传感器使用铜纳米线和石墨烯,有效地检测水和果汁中的pyrifos.
科学领域:
- 电化学 电化学 电化学
- 生物传感器是一种生物传感器.
- 纳米材料是一种纳米材料.
背景情况:
- 有机酸盐 (OPs) 具有重大环境和健康风险.
- 乙胆酶 (AChE) 抑制是OP毒性的关键机制.
- 对OPs开发敏感和选择性检测方法至关重要.
研究的目的:
- 开发一种酶电化学生物传感器,用于间接的OP检测.
- 为了提高生物传感器性能,使用铜纳米线/减少氧化石墨烯 (CuNWs/rGO) 纳米复合材料.
- 为了评估传感器在真实世界样本中检测pyrifos的有效性.
主要方法:
- 用CuNWs/rGO修改的印碳电极 (SPCE) 的制造.
- 使用乙胆酶 (AChE) 抑制作为检测原则.
- 通过基于 AChE 活动的循环电压计 (CV) 测量电化学信号.
主要成果:
- CuNWs/rGO纳米复合材料显著增强了信号电流和降低了氧化潜力.
- 生物传感器显示了pyrifos (10200μg/L) 的广泛线性检测范围.
- 达到低检测极限 (3.1微克/升) 和量化极限 (12.5微克/升).
结论:
- 开发的酶电化学生物传感器为OP检测提供了一种敏感和可靠的方法.
- CuNWs/rGO修改为电化学传感提供了增强的性能.
- 该传感器适用于高精度分析饮用水和汁等复杂矩阵中的pyrifos.
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