纳米秒脉冲电场 (nsPEF) 调节了等离子膜和线粒体中的电子运输
Kamal Asadipour1, Maisoun Bani Hani2, Lucas Potter1
1Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk Virginia, USA; Department of Electrical and Computer Engineering, Old Dominion University, Norfolk Virginia, USA.
Bioelectrochemistry (Amsterdam, Netherlands)
|September 22, 2023
概括
纳米秒脉冲电场 (nsPEF) 调节细胞中的电子运输. 低水平的nsPEF增强了这种传输,但在高水平时抑制了它,影响了细胞代谢和生存.
科学领域:
- 生物物理学的生物物理.
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 纳米秒脉冲电场 (nsPEFs) 是一种脉冲动力技术,具有已知的瘤切除能力.
- 除了瘤切除外,nsPEF还会影响各种生物机制.
- 血氧化还原系统 (PMRS) 和线粒体电子运输系统 (ETS) 对细胞的氧化还原稳定和新陈代谢至关重要.
研究的目的:
- 调查nsPEFs对PMRS中跨等离子膜电子传输 (tPMET) 的影响.
- 检查 nsPEF 对线粒体电子运输系统 (ETS) 活动的影响.
- 了解nsPEF如何调节活性氧物种 (ROS) 生产和细胞代谢.
主要方法:
- 在H9c2非癌性心脏肌细胞和4T1-luc乳腺癌细胞上利用nsPEF.
- 通过测量细胞透性WST-8四色素染料的减少来评估tPMET.
- 测量了线粒体的氧气消耗,以评估复合体I的ETS活动.
- 量化线粒体 (mROS) 和细胞质 (cROS) 活性氧物种.
主要成果:
- nsPEF对tPMET表现出双相效应:在较低的充电条件下增强,在较高的充电条件下抑制,表明hormesis.
- 复合体I的线粒体ETS活动被nsPEFs减弱.
- 在nsPEF处理下,ROS的产生在细胞类型和位置 (线粒体与细胞质) 之间存在差异.
- 较低的nsPEF水平支持糖解,而较高的水平抑制PMRS和线粒体ETS,导致细胞死亡.
结论:
- nsPEF显著调节tPMET和线粒体ETS活动,以剂量依赖的方式.
- 观察到的双相反应表明nsPEFs对细胞氧化还原功能的抑制作用.
- nsPEFs代表了一种用于操纵细胞氧化还原平衡,新陈代谢和潜在治疗癌症的新工具.
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