相关实验视频
Updated: Jul 16, 2025
06:16
mirMachine: A One-Stop Shop for Plant miRNA Annotation
Published on: May 1, 2021
2.6K
长非编码RNA (lnc663) 和微RNA (miR1128) 之间的相互作用调节面包小麦 (Triticum aestivum L.) 中的PDAT类基因活性
Akansha Madhawan1, Rupam Kumar Bhunia2, Prashant Kumar1
1Agri-Food Biotechnology Division, National Agri-Food Biotechnology Institute, Mohali, 140306, Punjab, India; Regional Centre for Biotechnology (RCB), NCR Biotech Science Cluster, 3rd Milestone, Faridabad-Gurgaon Expressway, Faridabad, Haryana, 121001, India.
Plant physiology and biochemistry : PPB
|September 22, 2023
概括
小麦粒脂质生物合成受TaPDAT的调节,TaPDAT是一种受Inc663和miR1128.8影响的基因. 这一发现提高了对抗性粉类型5 (RS-5) 和其健康益处的理解.
科学领域:
- 植物生物学 植物生物学
- 生物化学 生物化学
- 分子遗传学 分子遗传学
背景情况:
- 粉脂复合物形成耐性粉5型 (RS-5),有助于降低血糖指数和健康益处.
- 在小麦谷物中调节脂质生物合成的基因在很大程度上是未知的,这阻碍了对RS-5形成的理解.
- 了解这些基因对于提高谷物质量和营养价值至关重要.
研究的目的:
- 在小麦谷物中识别和表征脂质生物合成基因.
- 研究这些基因的转录后调节.
- 阐明控制小麦粒中的脂质积累的分子机制.
主要方法:
- 在小麦品种"C 306"和"TAC 75"中对粉结合脂质的定量分析.
- 小麦脂的基因表达分析:二甲基甘酸转移酶类 (PDAT类) 和调节性RNA (lnc663,miR1128).
- 过度表达,子宫外表达,酶活性测定和GFP记者测定以确认基因功能和调节.
主要成果:
- 小麦突变"TAC 75"的脂肪含量明显高于"C 306".
- 在"TAC 75"中,TaPDAT基因表达更高,其在三糖醇合成中的作用得到证实.
- lnc663对TaPDAT进行了积极调节,而miR1128则对其进行了负面调节,控制了脂质积累.
结论:
- TaPDAT 是小麦谷物脂质生物合成中的一个关键基因.
- lnc663和miR1128作为TaPDAT的关键调节剂,控制脂质的积累.
- 这项研究揭示了小麦粒脂质生物合成的新型调节机制,影响RS-5的形成.
相关概念视频
01:22MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
02:39lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
02:15Experimental RNAi
6.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.2K
01:23RNA Interference
26.1K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.1K
02:57piRNA - Piwi-interacting RNAs
6.9K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
6.9K
01:23Types of RNA
63.9K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
63.9K