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聚合物抑制复合体2沉积H3K27me3并抑制广泛的真核生物中的可转移元素
Tetsuya Hisanaga1, Facundo Romani2, Shuangyang Wu1
1Gregor Mendel Institute, Austrian Academy of Sciences, Vienna BioCenter, Dr. Bohr-Gasse 3, 1030 Vienna, Austria.
Current biology : CB
|September 22, 2023
概括
多镇压复合体2 (PRC2) 在多种类型的真核生物 (包括藻和红藻) 中使可转移元素 (TE) 沉默. 这种由PRC2进行的表观遗传沉默表明,在基因组调节中起到了古老的作用,可以追溯到早期的真核生物.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因组学就是基因组学.
- 进化生物学 进化生物学
背景情况:
- 可转移元素 (TE) 是可引起基因组不稳定的移动DNA序列.
- 主体基因组使用诸如DNA甲基化和H3K9甲基化之类的表观遗传标记来沉默TEs.
- 多胞体抑制复合体2 (PRC2) 沉积H3K27me3,这是一种与基因沉默相关的表观遗传标记,并与某些真核生物中的TE沉默有关.
研究的目的:
- 调查PRC2在不同真核细胞系中沉默可转移元素 (TE) 的更广泛作用.
- 为了确定PRC2-介导的H3K27me3沉积是否是一种在真核生物中TE沉默的保存机制.
主要方法:
- 在精选物种中产生了PRC2活性降低的突变.
- 分析了PRC2在TE中的作用和Archaeplastida和藻P. tricornutum*中的基因抑制.
- 检查了TE与邻近基因和开花植物中cis-regulatory元素的关联.
主要成果:
- 在藻中,PRC2抑制的TE比例高于藻P. tricornutum*和红藻C. merolae*的基因.
- 相比之下,PRC2抑制的基因比TEs在菌体中的比例更高.
- 在开花植物中,与邻近基因相关的TE被PRC2抑制,这表明它在塑造转录调节中的作用.
结论:
- 在藻,红藻,状藻类和Archaeplastida中,通过PRC2介导的TEs沉默是保留的.
- 在真核生物的最后一个共同祖先中,PRC2可能沉积了H3K27me3,以使TEs沉默.
- 由PRC2表观遗传标记的TE片段可能在Archaeplastida进化过程中被选择用于转录调节.
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