在OsCesA9中插入A Tos17转位子会导致米饭中的脆性
Hongzheng Sun1, Jiajun Sun1, Zeke Yuan1
1College of Agronomy, Henan Agricultural University, Zhengzhou 450046, PR China.
Gene
|September 22, 2023
概括
一种米突变,bc26,由于细胞壁组成的改变,表现出脆脆的茎. 这种脆弱的终点特征与Tos17转位子插入9.染色体Bc6基因的Tos17转位子有关.
科学领域:
- 植物遗传学 植物遗传学
- 分子生物学分子生物学
- 农业科学 农业科学
背景情况:
- 米茎的机械强度对于生长和产量至关重要.
- 在大米中的脆性 (bc) 突变体显示结构完整性降低.
- 了解易碎的基因基础对于作物改进至关重要.
研究的目的:
- 识别和表征一种新型的脆性突变在米中.
- 确定 bc26.26 中的脆性高峰表型的遗传和分子基础.
- 调查细胞壁组成对大米茎强度的作用.
主要方法:
- 对BC26突变和野生型大米的表型分析.
- 细胞壁成分 (纤维素,半纤维素) 的生物化学分析.
- 大量分离分析测序 (BSA-Seq) 来映射致病基因.
- Tos17转位子标记和测序以识别突变.
主要成果:
- bc26突变体表现出脆弱的叶子和茎,减少了植物的高度,以及更少的尖.
- bc26植物在各种组织中显示出明显较低的纤维素和较高的半纤维素含量.
- BSA-Seq将bc26基因定位到9号染色体,在Bc6基因中插入Tos17.
结论:
- 在bc26中,脆弱的顶点特征是由Tos17转位子插入Bc6基因引起的.
- 这种插入可能导致Bc6基因功能丧失,影响细胞壁生物合成.
- bc26代表了BC6基因的新等位基因,为米的机械强度调节提供了洞察力.
相关概念视频
Overview of Transposition and Recombination
15.6K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
15.6K
DNA-only Transposons
14.6K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
The donor site from where the transposon is excised is either degraded or...
14.6K
Transposons
52
Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...
52
Transgenic Plants
7.3K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.3K


