使用代谢建模分析Pichia pastoris生长合的血红素生物合成分析
Agris Pentjuss1,2, Emils Bolmanis3, Anastasija Suleiko4,5
1Microbiology and Biotechnology Institute, University of Latvia, Jelgavas Street 1, Riga, 1004, Latvia. agris.pentjuss@gmail.com.
Scientific reports
|September 22, 2023
概括
这项研究优化了Pichia pastoris在植物性肉类中的血生产. 代谢建模确定了通过增强糖醇培养和调节代谢基因来增加血球产量的策略.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 食品科学 食品科学 食品科学
背景情况:
- 豆血红蛋白对于植物性肉的颜色和味道至关重要.
- 对于食品工业来说,高产的血红蛋白及其血红成分的生产是必不可少的.
研究的目的:
- 为了增强Pichia pastoris中高产的血红蛋白和血红蛋白的生产.
- 确定最佳的种植条件和代谢工程策略,以增加血的生产.
主要方法:
- 在糖醇和甲醇上种植Pichia pastoris.
- 在体内代谢建模用于增长合酶数量分析.
- 鉴定基因上下调节策略,以优化代谢.
主要成果:
- 糖种植显示了吸收和生产率增加50%的潜力.
- 甲醇种植接近了理论上的最大值.
- 代谢建模表明,33个反应用于上调和66个反应用于下调/删除,以优化血生产.
结论:
- 代谢建模为改善Pichia pastoris.的血红素生产提供了有效的策略.
- 优化种植和基因调节可以显著提高肉类替代品的血红蛋白产量.
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