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维生素D3调节PM驱动的人类初级中性粒细胞的炎症反应

Chidchamai Kewcharoenwong^1,2, Aranya Khongmee², Arnone Nithichanon^2,3

¹Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, 50200, Thailand.

Scientific reports

|September 22, 2023

概括

颗粒物 (PM) 空气污染会损害中性粒细胞的功能和杀死细菌. 维生素D补充剂可以减轻维生素D缺乏症患者的这些不良影响.

科学领域:

免疫学免疫学免疫学
环境健康环境健康
毒理学毒理学毒理学

背景情况:

颗粒物 (PM) 空气污染与呼吸道感染和败血症死亡率有关.
暴露于颗粒物可能会对中性粒细胞功能产生不利影响,中性粒细胞功能是先天免疫的关键组成部分.
迫切需要策略来减轻PM对免疫细胞的影响.

研究的目的:

研究城市PM和PM2.5对人类中性粒细胞功能的影响.
评估维生素D在调节PM诱导的中性粒细胞功能障碍中的作用.
探索维生素D补充作为一种潜在的缓解策略.

主要方法:

人类周围血液中性粒细胞暴露于来自泰国的标准城市PM (SRM1648a,NIST) 和PM2.5.
评估的中性粒细胞功能包括IL-8的产生,CXCR2的表达,迁移和杀死细菌.
在足够和不足的维生素D状态下,在体外维生素D3补充剂中评估了效果.

主要成果:

这两种PM类型都增加了IL-8的产生,但减少了CXCR2表达和LPS诱导的迁移.
来自维生素D不足个体的暴露在PM中的中性粒细胞显示出受损的大肠杆菌杀伤.
在体外维生素D3补充剂改善了细菌的杀死,并减少了这些细胞中的IL-8产量.

结论:

环境中的颗粒物暴露会对人类中性粒细胞功能产生负面影响,包括迁移和细菌清除.
维生素D不足会加剧PM诱导的中性粒细胞功能障碍.
补充维生素D有望减轻对PM的不良中性友好反应.

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