曲线诱导的结构变化,由单壁碳纳米管的毛头部的子域
Srijita Paul1, Parbati Biswas1
1Department of Chemistry, University of Delhi, Delhi, India. pbiswas@chemistry.du.ac.in.
Physical chemistry chemical physics : PCCP
|September 23, 2023
概括
碳纳米管 (CNT) 显示出药物输送的前景. 它们的曲率影响蛋白质吸附,更大的直径增加结合,但达到极限,而释放需要特定的力和速度来保持蛋白质的完整性.
科学领域:
- 生物材料科学 生物材料科学
- 纳米技术纳米技术
- 计算化学计算化学
背景情况:
- 碳纳米管 (CNTs) 正在探索药物和生物分子输送.
- CNT的性决定了直径,影响了生物分子的相互作用.
- 蛋白质-纳米管相互作用对于有效的输送系统至关重要.
研究的目的:
- 研究蛋白质和CNT之间的依赖曲率的相互作用.
- 确定CNT直径对蛋白质吸附和封装的影响.
- 分析 CNTs 释放蛋白质所需的力和速度.
主要方法:
- 经典的分子动力学模拟.
- 导向的分子动力学模拟.
- 模拟皮头饰子域 (HP36) 和不同奇拉度的CNT之间的相互作用.
主要成果:
- 蛋白质吸附强度随着CNT半径增加到和点.
- 在 (28,28) CNT中自发封装HP36,结构变化最小.
- 蛋白质释放而不展开所需的最小力为~300 pN和速度为4 Å ns-1.
结论:
- CNT曲线显著影响蛋白质吸附和封装.
- 控制释放参数对于保护蛋白质结构和功能至关重要.
- 这些发现有助于设计基于CNT的药物输送系统.
相关概念视频
Additional Subnuclear Structures
4.6K
The eukaryotic nucleus is a double membrane-bound organelle that contains nearly all of the cell’s genetic material in the form of chromosomes. It is rightly called the “brain” of the cell as it shoulders the responsibility of responding to various physiological processes, stress, altered metabolic conditions, and other cellular signals.
The nucleus contains many membrane-less subnuclear organelles or nuclear bodies, such as nucleoli, Cajal bodies, speckles,...
The nucleus contains many membrane-less subnuclear organelles or nuclear bodies, such as nucleoli, Cajal bodies, speckles,...
4.6K
Disassembly of Intermediate Filaments
2.1K
Intermediate filaments (IFs) do not undergo spontaneous disassembly. Enzymes, kinases, and phosphatases add and remove phosphates from specific sites to regulate their disassembly. The IF concentration in the cytoplasm also regulates the disassembly. If the concentration crosses a threshold, it activates the protein kinases in the vicinity, allowing the phosphorylation of IFs.
Keratin proteins, found at the cell periphery near cell junctions, undergo a cycle of assembly and disassembly. In Type...
Keratin proteins, found at the cell periphery near cell junctions, undergo a cycle of assembly and disassembly. In Type...
2.1K
Studying the Cytoskeleton
6.3K
The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
6.3K
Globular and Fibrous Proteins
43.8K
Many proteins can be classified into two distinct subtypes - globular or fibrous. These two types differ in their shapes and solubilities.
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...
43.8K


