使用液体染色学-并联质谱学进行二维异构体差异化,采用源内,基于滴滴的衍生化
Derik R Heiss1,2, Enoch Amoah1, Abraham K Badu-Tawiah1
1Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA. badu-tawiah.1@osu.edu.
The Analyst
|September 23, 2023
概括
这项研究引入了一种新方法,将液态色谱与质谱相结合,用于分析糖异构体. 该技术使用即时衍生物来区分复杂的糖类,改善生物标志物和治疗应用.
科学领域:
- 分析化学 分析化学
- 生物化学 生化学
- 质谱测量质量谱测量
背景情况:
- 糖类是重要的生物标志物和治疗药物.
- 由于电离效率低和结构复杂,鉴定糖类的特征是很困难的.
- 现有的方法难以区分糖异构体.
研究的目的:
- 开发一种全面的方法来表征糖糖同位素.
- 为了能够区分传统色谱学或质谱学无法区分的糖类物种.
- 在蜂蜜样本中应用分析糖的方法.
主要方法:
- 使用含有电子喷雾 (ES) 离子源的在线液滴反应与液态染色学 (LC) 协同质谱学 (MS/MS) 的合.
- 使用酸和cis-diols的糖的飞行衍生物化.
- 在现场MS/MS分析用于生成诊断断片离子.
主要成果:
- 用LC-contained-ES-MS/MS方法成功地分化了含有微妙染色学差异的糖糖异构体.
- 诊断断片离子使得以前仅用LC或MS无法区分的物种可以被识别出来.
- 该方法证实了蜂蜜样本中图拉诺, palatinose, maltulose 和 maltose 的存在.
结论:
- 开发的直角LC-contained-ES-MS/MS工作流提供了一个强大的工具,用于全面的糖类特性.
- 这种方法增强了复杂碳水化合物混合物的分析,包括异构体.
- 该方法可适应现有的质谱仪,并显示出在糖类分析中广泛应用的前景.
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