将WALDI和MALDI对脂质的MS成像进行比较:两种技术用于评估MS成像中的共同基础真相
Léa Ledoux1, Yanis Zirem1, Florence Renaud2
1Univ. Lille, Inserm, CHU Lille, U1192 - Protéomique Réponse Inflammatoire Spectrométrie de Masse - PRISM, F-59000 Lille, France. isabelle.fournier@univ-lille.fr.
The Analyst
|September 23, 2023
概括
水辅助激光脱电离 (WALDI) 和矩阵辅助激光脱电离 (MALDI) 质谱成像显示在组织分析中具有非常相似的脂质特征. 这种一致性允许将两种技术的数据集结合起来,以增强生物医学研究模型.
科学领域:
- 生物医学研究生物医学研究
- 分析化学 分析化学
- 质谱仪成像成像 质谱仪成像
背景情况:
- 矩阵辅助激光脱吸电离 (MALDI) 是无标签分子组织分析的基准.
- 水辅助激光脱吸电离 (WALDI) 为类似的分析提供了替代方法.
研究的目的:
- 为了直接比较WALDI和MALDI质谱成像 (MSI) 用于脂质学分析.
- 评估通过这两种技术获得的脂质学数据的相似性和相关性.
主要方法:
- 在生物样本上直接比较WALDI-MSI和MALDI-MSI.
- 计算峰值相似性和皮尔森光谱强度的相关性.
- 在负离子和正离子模式中使用诺哈曼作为MALDI矩阵的研究.
主要成果:
- 在WALDI-MSI和MALDI-MSI脂质组分析之间具有很高的整体相似性.
- 100%的峰值相似性和>0.90强度相关性对于负离子模式中的诺哈曼.
- 阳离子模式光谱的峰值相似度超过83%,由于WALDI光谱较丰富,WALDI-MSI与MALDI-MSI进行比较时为77%.
结论:
- 沃尔迪-MSI和马尔迪-MSI提供一致的脂质组概况,由类似的分析过程来管理.
- 高一致性使得两种模式的数据集可以结合起来.
- 结合的数据集可以改善分类模型的培训,并促进生物医学研究中的交叉查询.
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