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从爆炸物污染的环境中分离出来的细菌在有氧和无氧条件下转化甲四酸 (PETN)

Helena Avila-Arias¹, Francy-Carolina Casallas¹, Ziv Arbeli¹

¹Unidad de Saneamiento y Biotecnología Ambiental (USBA), Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá 110231, Colombia.

Letters in applied microbiology

|September 23, 2023

概括

研究人员分离了12种能够转化爆炸性Pentaerythritol tetranitrate (PETN) 的细菌培养物. 这些微生物在多种类型中发现,显示出 PETN 污染环境的生物修复潜力.

科学领域:

环境微生物学环境微生物学
生物修复是一种生物修复.
化学工程是化学工程的组成部分.

背景情况:

甲四酸 (PETN) 是一种持久性爆炸物,对其环境命运的数据有限.
了解微生物转化途径对于开发补救策略至关重要.

研究的目的:

在有氧和无氧条件下分离和描述能够转化PETN的细菌.
为了评估PETN生物转化带有和没有额外的碳来源的效率.

主要方法:

在爆炸性制造工厂从环境样本中分离PETN转化细菌.
在有氧 (含/不含碳) 和无氧 (含碳) 条件下用PETN (100 mg L-1) 化培养物.
量化PETN转化,化物释放,细菌生长和代谢物形成.

主要成果:

分离了12种PETN转化培养 (4种纯种,8种共同培养).
最高的PETN转化 (91%±2%在2天内) 发生在与碳源的有氧过程中,与酸盐释放和细菌生长有关.
无氧转化达到52%±13%,形成了两个未知的代谢物.
转化PETN的细菌属于Alphaproteobacteria,Betaproteobacteria,Gammaproteobacteria和Actinobacteria的细菌.这些细菌可以被分为三种类型.

结论:

通过各种细菌种群,可以实现PETN转化.
有碳源的有氧条件增强了PETN的生物降解.
孤立的培养表明了对PETN污染地区的生物修复的潜力.

关键词:

亚格罗细菌 sp 的种类.布拉迪瑞佐比乌姆 sp sp 的一种.罗多科克斯 sp sp 在这里.在PETN生物转化过程中.异生生物的异生生物.

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