氨酸生物合成是由Aureobasidium melanogenum中自生宿主酸激活引起的
Mei Zhang1, Xin Wei1, Peng Wang1
1College of Marine Life Science, Ocean University of China, Qingdao, China.
Biotechnology journal
|September 23, 2023
概括
由于分泌的酸引起的环境pH值较低,增强了氨酸的产生. 这通过PacC转录因子发生,该转录因子激活了氨酸生物合成的关键基因,克服了抑制.
科学领域:
- 微生物的新陈代谢
- 生物化学途径的使用
- 基因调节 基因调节
背景情况:
- 最大限度的氨酸生产需要较低的环境pH值 (~3.0).
- 酸分泌是这种低pH的主要原因.
- 酸是酸-CoA在氨酸生物合成中的前体.
研究的目的:
- 阐明酸和pH在氨酸生物合成中的作用.
- 确定控制氨酸生产的监管机制.
- 了解PACC基因的功能及其与氨酸合成的关系.
主要方法:
- 在培养中定量酸.
- 基因删除,补充和过度表达 (CEXA,ACL,PACC).
- 在不同的pH值和酸条件下对基因表达的分析.
主要成果:
- 经CEXA确认的酸分泌会导致低pH.
- 已证明低pH和酸有助于细胞内酸溶解和氨酸的产生.
- 确定了PACC作为一种调节氨酸生物合成的酸表达基因基因集群 (GAL1-EST1-PKS1).
- 观察到在低pH下对氨酸生物合成的弱催化剂抑制.
结论:
- 由于自身宿主酸激活和PacC pH信号通路,氨酸生物合成在低pH下被诱导.
- 该PacC转录因子直接调节氨酸生物合成基因集群.
- 提议增强氨酸生物合成的机制,涉及pH信号和宿主酸激活.
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