来自不同来源的β-葡萄糖在便细菌在体外发酵前和后具有抗炎活性
Jing Cheng1, Guangwen Zhang1, Liu Liu1
1Department of Food Science and Engineering, Jinan University, Guangzhou, China.
Journal of the science of food and agriculture
|September 23, 2023
概括
大麦β-葡萄糖表现出强大的抗炎作用,优于其他来源. 发酵增强了这种活性,特定的代谢物被确定为评估抗炎性质的潜在生物标志物.
科学领域:
- 生物化学 生化学
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
背景情况:
- β-葡萄糖具有多种生理作用,特别是抗炎性质.
- 识别强效的β-葡萄糖和可靠的生物标志物对其活性至关重要.
- 这项研究解决了选β-葡萄糖抗炎功效的必要性,并发现相关的代谢生物标志物.
研究的目的:
- 来自五个不同来源的β-葡萄糖选其抗炎活性.
- 在肠道微生物代谢物中识别与抗炎作用相关的潜在生物标志物.
- 研究肠道微生物发酵对β-葡萄糖抗炎潜力的影响.
主要方法:
- 评估脂聚糖 (LPS) 诱导的促炎媒介和TLR4/MyD88的mRNA表达的抑制.
- 评估细胞内活性氧物种 (ROS) 生产和NF-κB/JNK通路活性.
- 通过肠道微生物进行β-葡萄糖发酵后分析代谢物概况和细菌丰度.
主要成果:
- 所有测试的β-葡萄糖通过抑制促炎介质和相关信号通路,都显示出抗炎作用.
- 大麦β-葡萄糖表现出最强烈的抗炎活性,其次是Ganoderma lucidumβ-葡萄糖.
- 发酵的β-葡萄糖表现出增强的活性,与增加的香草素,二基酸,咖啡酸,酸,黄油酸和乳酸水平有关,与像Blautia这样的有益细菌相关.
结论:
- 大麦是制备具有显著抗炎性质的β-葡萄糖的优质来源.
- 包括香草素,二基酸,咖啡酸,酸,黄油酸和乳酸在内的特定代谢物被提议作为评估β-葡萄糖抗炎功能的生物标志物.
- 这些发现为开发使用发酵β-葡萄糖的向抗炎策略提供了基础.
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