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Xiawan Zheng¹, Keyue Zhao¹, Xiaoqing Xu¹

¹Environmental Microbiome Engineering and Biotechnology Lab, Center for Environmental Engineering Research, Department of Civil Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, China.

|September 23, 2023

概括

废水监测有效追踪甲型和乙型流感病毒 (IAV/IBV),补充临床数据. 开发的协议显示IAV水平与疾病相关,有助于公共卫生反应.

科学领域:

环境微生物学环境微生物学
公共卫生监督是对公共卫生的监督.
病毒学病毒学

背景情况:

废水监测为SARS-CoV-2和流感等传染病提供了具有成本效益的近实时监测.
仅靠临床监测可能无法捕捉到病毒在整个人口中传播的全部动态.

研究的目的:

开发和验证废水中流感A和B病毒 (IAV/IBV) 监测的预分析协议.
在香港的废水网络中实施IAV/IBV的大规模纵向监测.
为了将废水病毒度与临床监测数据和公共卫生措施相关联.

主要方法:

为了检测IAV/IBV,开发了两种预分析协议 (基于超剂和基于颗粒).
来自24个地点的724个废水样本 (每周一次) 和来自11个WWTP的458个样本 (每周3次) 分别在8个月和4个月内被分析.
病毒亚型识别和桑格测序用于确认.

主要成果:

全市IAV废水度与临床检测率和类似流感的疾病加 (ILI+) 率相关.
在面具任务取消后,IAV水平显著增加.
IBV的检测率较低,与临床监测结果一致.
H1pdm被确定为主要流通的IAV亚型.
桑格测序证实了基于废水的病毒检测的准确性.

结论:

IAV/IBV废水监测适用于现有基础设施.
这种方法提供了一种快速,具有成本效益的策略,用于跟踪社区病毒传播.
废水监测可以为及时的公共卫生行动和未来的流行病准备提供信息.

关键词:

流感病毒是流感病毒.在PEG降水量下降.RT-qPCRR 是一个人.病毒的爆发病毒的爆发.废水监测废水监测废水监测废水监测

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