相关实验视频

Updated: Jul 15, 2025

Purification and Transplantation of Myogenic Progenitor Cell Derived Exosomes to Improve Cardiac Function in Duchenne Muscular Dystrophic Mice

Purification and Transplantation of Myogenic Progenitor Cell Derived Exosomes to Improve Cardiac Function in Duchenne Muscular Dystrophic Mice

Published on: April 10, 2019

SOX4作为病理性心脏缩的潜在治疗标

Shen Bin¹, Feng Xinyi¹, Pan Huan²

¹Department of Pharmacology, College of Medical, Jiaxing University, Jiaxing, 314000, China.

European journal of pharmacology

|September 23, 2023

概括

SOX4 (SRY-box 4) 通过抑制SIRT3,增加氧化应激,促进病态心脏缩. 减少SOX4表达可能为预防心力衰竭提供一种新的治疗策略.

科学领域:

心脏病学心脏病学
分子生物学分子生物学
生物化学生物化学

背景情况:

病理性心脏缩是心力衰竭的先驱,需要采取预防策略.
转录因子SOX4 (SRY-box 4) 在心脏缩中的作用在很大程度上仍未定义.
了解驱动心脏缩的分子机制对于开发向疗法至关重要.

研究的目的:

研究SOX4在病理性心脏缩中的作用和机制.
为了确定SOX4是否影响心肌细胞中的氧化应激通路.
评估SOX4作为心脏缩的潜在治疗标.

主要方法:

评估SOX4表达在高变性心脏模型和血管新生素II治疗新生小鼠心肌细胞 (NRCMs) 中.
在NRCM和小鼠心脏中利用SOX4的淘汰技术来评估多变性反应.
使用分子生物学试验研究了SOX4和SIRT3促进体之间的分子相互作用.
测量了活性氧物种 (ROS) 和MnSOD乙化水平,以评估氧化应激.

主要成果:

SOX4表达在过度缩的心脏和Ang II刺激的NRCMs中显著上调.
在细胞和体内模型中对SOX4减弱心脏缩的击败.
SOX4直接与SIRT3促进体结合,抑制其转录和表达.
抑制SOX4导致减少MnSOD乙化,减少ROS产量,并减轻氧化应激.

结论:

SOX4在促进病理性心脏缩方面发挥着关键作用.
该机制涉及SOX4介导的SIRT3抑制,导致氧化应激增加.
准SOX4为治疗心脏缩和预防心力衰竭提供了一个有希望的治疗途径.

关键词:

病理性心脏缩症病理性心脏缩症在SIRT3中,SIRT3是最重要的.这就是SOX4的SOX4.

更多相关视频

Cardiac Spheroids as in vitro Bioengineered Heart Tissues to Study Human Heart Pathophysiology

Cardiac Spheroids as in vitro Bioengineered Heart Tissues to Study Human Heart Pathophysiology

Published on: January 23, 2021

Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart

Light-sheet Fluorescence Microscopy to Capture 4-Dimensional Images of the Effects of Modulating Shear Stress on the Developing Zebrafish Heart

Published on: August 10, 2018

相关概念视频

Pleiotropy

Pleiotropy

Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...

Cardiomyopathy III: Hypertrophic Cardiomyopathy

Cardiomyopathy III: Hypertrophic Cardiomyopathy

Hypertrophic cardiomyopathy, or HCM, is an autosomal dominant genetic disorder characterized by asymmetric left ventricular hypertrophy without ventricular dilation. It is more common in men and is typically diagnosed in young, athletic adults.EtiologyHCM is primarily genetic and is caused by mutations in genes encoding sarcomeric proteins. Researchers have identified over 1400 mutations across at least 11 different genes. Among these, the most frequently occurring mutations are found in the...

Cardiomyopathy IV: Restrictive Cardiomyopathy

Cardiomyopathy IV: Restrictive Cardiomyopathy

Restrictive cardiomyopathy (RCM) is a rare heart muscle disease characterized by impaired ventricular filling due to stiffened ventricular walls, leading to significant diastolic dysfunction.EtiologyRestrictive cardiomyopathy can arise from both inherited and acquired diseases, many of which are systemic. It is categorized into four main types: infiltrative, storage, non-infiltrative, and endomyocardial diseases.Infiltrative diseases, such as amyloidosis, lead to RCM by depositing amyloid...