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在人类梅博米腺上皮细胞中比较不同的脂质染色方法
Yang Liu1, Shan Yang2, Xiaomin Chen1
1Department of Ophthalmology, Zhongnan Hospital of Wuhan University, Wuhan, China.
Experimental eye research
|September 23, 2023
概括
四种染料在人类梅博米腺细胞中进行了细胞内脂质检测的测试. 油红O,苏丹III和LipidTOX绿色有效地识别了在分化过程中的脂质积累,与尼罗河红色不同.
科学领域:
- 细胞生物学 细胞生物学
- 眼睛表面研究 眼睛表面研究
- 脂质代谢 脂质代谢是什么
背景情况:
- 细胞内脂质积累对于梅博米腺上皮细胞分化至关重要.
- 需要准确的检测方法来研究人类梅博米腺上皮细胞 (IHMGECs) 中的脂质变化.
研究的目的:
- 为了比较四种常见染料 (Oil Red O,Sudan III,LipidTOX绿色,尼罗河红色) 在IHMGEC中检测细胞内脂质的疗效.
- 为了评估这些染料如何显示由Roxadustat诱导的脂质变化,一个差异化剂.
主要方法:
- 为了诱导分化,IHMGECs被培养并与Roxadustat或没有Roxadustat一起处理.
- 细胞使用Oil Red O,Sudan III,LipidTOX绿色或尼罗河红色进行染色.
- 使用显微镜和ImageJ软件量化了脂质囊泡的大小,数量,面积和染色强度.
主要成果:
- 油红O和苏丹III在接受Roxadustat治疗的细胞中增加了脂质囊泡的大小和面积,而油红O显示出比苏丹III更大的囊泡.
- LipidTOX绿色表示细胞内脂质强度增加,而尼罗河红色没有显示显著变化.
- 所有测试的染料都显示了脂质模式的差异,但油红O,苏丹III和LipidTOX绿色比尼罗河红更有效.
结论:
- 油红O,苏丹III和LipidTOX绿色适用于量化细胞内脂质积累,以区分IHMGECs.
- 尼罗河红色在检测Roxadustat诱导的脂质变化方面效率较低,在这种细胞模型中.
- 染料选择对IHMGECs中脂质动态的评估产生重大影响.
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