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黑木提取物通过T2DM小鼠的AMPK/mTOR信号通路抑制肝脏脂肪生成

Yudi Yao¹, Yang Chen², Huijian Chen¹

¹School of Pharmaceutical Sciences, South-Central Minzu University, Wuhan, 430074, China.

Journal of ethnopharmacology

|September 23, 2023

概括

黑果提取物 (MNF-EA) 在治疗2型糖尿病 (T2DM) 方面表现有前途. 它通过AMPK/mTOR途径调节肝脂代谢,有效降低血糖并改善胰岛素抵抗.

科学领域:

药理学药理学是指药理学的学科.
代谢障碍代谢障碍代谢障碍
自然产品自然产品

背景情况:

黑 (Morus nigra L.) 传统上是因为它的低血糖特性而被使用的.
精确的机制,特别是关于肝脏脂质积累的机制,在糖尿病治疗中仍未得到充分研究.

研究的目的:

为了研究Morus nigra L.果实乙烯酸提取物 (MNF-EA) 降低血糖的功效.
确定MNF-EA是否能预防肝脏脂质生成,并作为2型糖尿病 (T2DM) 的辅助疗法.

主要方法:

使用UPLC-Q-TOF-MS进行MNF-EA的化学成分分析.
在体外研究使用HepG2-IR和OA诱导的HepG2细胞 (MTT,葡萄糖吸收,油红O,西部斑).
在STZ-HFD联合诱导T2DM小鼠的体内研究 (指数,生物化学分析,组织病理学,西部斑).

主要成果:

MNF-EA确定了19种化合物,并改善了HepG2细胞中的胰岛素抵抗和脂质滴积,没有副作用.
在T2DM小鼠中,MNF-EA降低了禁食血糖,改善了葡萄糖耐受性和胰岛素耐药性,改善了脂质代谢障碍和肝功能.
组织病理学揭示了减少的肝肥胖症,机理学研究指出AMPK/mTOR途径.

结论:

通过调节AMPK/mTOR信号通路,MNF-EA显示出扭转T2DM代谢异常的潜力.
黑色 L. 是一个有前途的药物和食品同源剂,用于改善T2DM.
对MNF-EA作为T2DM辅助疗法的进一步研究是有必要的.

关键词:

5′-腺单酸盐激活蛋白激酶 (AMPK) 的作用.肝脏肥胖症肝脏肥胖症猛素的哺乳动物标 (mTOR)黑色毛虫 L. 黑色毛虫 L.2 型糖尿病 2 型糖尿病

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