相关实验视频

Updated: Jul 15, 2025

Two Flow Cytometric Approaches of NKG2D Ligand Surface Detection to Distinguish Stem Cells from Bulk Subpopulations in Acute Myeloid Leukemia

Two Flow Cytometric Approaches of NKG2D Ligand Surface Detection to Distinguish Stem Cells from Bulk Subpopulations in Acute Myeloid Leukemia

Published on: February 21, 2021

NKG2D受体通过与青少年异常性关节炎患者的树突细胞相互作用来调节CD4⁺T细胞分化

Juan Zhou¹, Junyan Wang², Linlin Tao³

¹Department of Pediatric Allergy, Immunology & Rheumatology, Guangzhou Women and Children's Medical Center, Guangdong, Provincial Clinical Research Center for Child Health, Guangzhou, China.

Clinical immunology (Orlando, Fla.)

|September 23, 2023

概括

自然杀手组2D (NKG2D) 受体在青少年异常性关节炎 (JIA) 中发挥着关键作用. 它影响T细胞激活和树突细胞成熟,影响疾病活动和亚型.

科学领域:

免疫学免疫学免疫学
类风湿病学类风湿病学
细胞生物学细胞生物学

背景情况:

自然杀手组2D (NKG2D) 是一种提供T细胞激活共刺激信号的受体.
它在青少年异常性关节炎 (JIA) 中CD4+T细胞和树突细胞 (DCs) 之间的相互作用中的确切作用仍然不完全理解.

研究的目的:

在JIA的背景下,研究NKG2D在CD4+T细胞和DC相互作用中的功能.
阐明NKG2D信号如何影响JIA患者的T细胞分化和DC成熟.

主要方法:

使用NKG2D转基因CD4+T细胞进行实验分析.
在JIA患者 (关节和全身亚型) 和健康对照者之间,对T细胞的NKG2D表达和DC上的MICA/MICB表达进行了比较.
在CD4+T细胞中评估了细胞因子 (IL-12,IL-10,TGF-β,IFN-γ,IL-17,IL-4) 和转录因子表达 (RORc,T-bet,Foxp3,GATA3).
评估的直流成熟标记 (CD83) 和配体表达 (MICA,MICB).

主要成果:

与健康对照组相比,活跃的JIA患者表现出CD4+NKG2D+T细胞的频率显著更高.
在关节性JIA和系统性JIA中观察到CD4+ T细胞的NKG2D表达升高和DCs上的MICA/MICB表达.
在CD4+T细胞中NKG2D信号促进了IL-12的产生,抑制了IL-10和TGF-β,并通过上调RORc和T-bet来偏向IFN-γ+和IL-17+T细胞的分化,同时降低了调控性T细胞标记物 (CD25,Foxp3) 和IL-4的产生.
在DC上NKG2D信号导致IL-10降低,并增加CD83,MICA和MICB表达在JIA患者和对照组中.

结论:

在JIA中,NKG2D直接调节CD4+T细胞分化.
NKG2D间接影响树突细胞成熟,这表明它在调节JIA病原体内的免疫反应方面发挥了双重作用.

关键词:

CD4 (((+) T 细胞细胞树突细胞的树突细胞.不同化的差异化青少年无形性关节炎 (Juvenile idiopathic arthritis) 是一种不良的疾病.在NKG2D中,它是NKG2D.

更多相关视频

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Published on: September 26, 2013

Development and Functional Characterization of Murine Tolerogenic Dendritic Cells

Development and Functional Characterization of Murine Tolerogenic Dendritic Cells

Published on: May 18, 2018

相关概念视频

T Cell Types and Functions

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...

T Cell Activation and Clonal Selection

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...