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模式识别受体参与了鱼 (Eptatretus burgeri) 的免疫系统

Kenya Arata¹, Tomokazu Yamaguchi¹, Kazufumi Takamune²

¹Department of Biological Science, Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto, 860-8555, Japan.

Developmental and comparative immunology

|September 23, 2023

概括

鱼GlcNAc识别复合体 (GRC) 在先天免疫系统中作为模式识别受体 (PRR) 起作用,激活巨细胞灭菌. 它的31kDa蛋白质与可变淋巴细胞受体-B (VLR-B) 具有结构上的相似性.

科学领域:

免疫学免疫学免疫学
生物化学生物化学
进化生物学进化生物学

背景情况:

天生的免疫依赖于模式识别受体 (PRRs) 结合病原体相关的分子模式 (PAMPs).
像鱼这样的无脊椎动物提供了关于脊椎动物早期免疫进化的见解.

研究的目的:

从鱼血清中鉴定GlcNAc识别复合体 (GRC) 的特征.
调查鱼GRC作为潜在的PRR的功能和结构特性.

主要方法:

使用GlcNAc-agarose从鱼血清中净化GRC.
在接触大肠杆菌和GRC时评估巨细胞细胞激活.
确定GRC的分子质量和结合性质.
分析31 kDa蛋白成分的序列和免疫反应.

主要成果:

鱼GRC由四种蛋白质 (19,26,27,31kDa) 组成,激活大肠杆菌的巨细胞化,这表明它具有声功能.
GRC形成了一个大型血清复合体 (~1200 kDa),并特别结合大肠杆菌,而不是子的红细胞.
这种31kDa的蛋白与鱼变性淋巴细胞受体-B (VLR-B) 具有序列相似性,并被抗-VLR-B抗血清识别.

结论:

鱼GRC的功能是PRR,类似于其他脊椎动物的曼诺结合性讲解蛋白 (MBL).
GRC的31kDa成分与VLR-B具有结构同质性,这表明无鱼的先天性和适应性免疫成分之间存在联系.

关键词:

GlcNAc的识别复合体这是天生的免疫力.没有的脊椎动物鱼.模式识别受体是一种模式识别受体.

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