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无领导通信 (LCP) 类的定数感应在Firmicutes中广泛分布
Shifu Aggarwal1,2, Elaine Huang1,2, Hackwon Do1,2,3
1Center for Molecular and Translational Human Infectious Diseases Research, Houston Methodist Research Institute, Houston, TX, 77030, USA.
Nature communications
|September 23, 2023
概括
细菌使用无领导通信 (LCPs) 进行细胞间信号传递和毒性控制. 这项研究揭示了LCP在Firmicutes中广泛存在,在各种物种中调节基因表达.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 细菌遗传学 细菌遗传学
背景情况:
- 斯杆菌pyogenes利用无领导通信 (LCPs) 通过RopB受体进行细胞间通信和毒性调节.
- 除了S. pyogenes之外,LCPs的流行和功能尚未得到充分理解.
研究的目的:
- 调查LCP及其同类在Firmicutes属内的其他细菌物种中的存在和功能.
- 实验验证LCPs作为调节基因表达的细胞间信号的作用.
主要方法:
- 生物信息分析以确定各种Firmicutes物种中的LCP和RopB同类物种.
- 在Streptococcus salivarius,Streptococcus porcinus,Enterococcus malodoratus和Limosilactobacillus reuteri.中对LCP功能进行实验验证.
主要成果:
- 在多个Firmicutes物种中确定了LCP和RopB同类物种,这表明其分布比以前知道的更广泛.
- 实验数据证实,不同的LCPs作为细胞间信号起作用,调节被测试物种中的基因表达.
- 这些发现突显了LCP介导的信号传递在多种细菌基因中的保存性.
结论:
- 无领导的沟通 (LCP) 是一种广泛的细菌细胞间信号分子类,属于Firmicutes.
- 对LCPs的表征提供了对新型信号机制及其在协调细菌特征中的作用的见解.
- 这项研究扩大了我们对细菌交流及其对毒性和基因调节的影响的理解.
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