在患有结性脊柱炎的中国患者中,PRICKLE1基因甲基化和异常转录
Tingting Zhou1, Xinqi Wang1, Jiangping Kong1
1Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230032, China; The Key Laboratory of Major Autoimmune Diseases, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230032, China.
性脊柱炎患者的PRICKLE1基因甲基化减少和mRNA水平增加. 这表明PRICKLE1可能在AS病变发生过程中发挥作用,并可以作为早期诊断生物标志物.
科学领域:
- 遗传学和分子生物学
- 免疫学 免疫学 免疫学
- 类风湿病学 类风湿病学
背景情况:
- 结节性脊柱炎 (AS) 是一种普遍的炎症性关节炎,缺乏可靠的生物标志物.
- 在AS病变发生过程中,PRICKLE1促进体甲基化和mRNA表达的具体作用尚不清楚.
研究的目的:
- 为了研究PRICKLE1基因的甲基化状态和mRNA表达在患有结性脊髓炎的患者.
- 确定PRICKLE1是否可以作为AS的潜在生物标志物.
主要方法:
- 采用了两阶段病例控制研究设计.
- 使用酸盐测序分析了PRICKLE1基因促进物甲基化.
- 在50名AS患者和50名健康对照中,通过实时定量PCR (RT-qPCR) 量化PRICKLE1mRNA表达水平.
主要成果:
- 与健康对照组相比,AS患者的PRICKLE1基因促进物甲基化显著降低.
- 在AS患者中观察到PRICKLE1mRNA的过度表达 (P = 0.017).
- 接收器操作特征 (ROC) 分析显示,PRICKLE1.1.的灵敏度为68.67%,特异性为71.43%.
结论:
- 在AS患者中,PRICKLE1mRNA度的显著改变和基因甲基化减少是显而易见的.
- 这些发现表明PRICKLE1基因参与AS病变发生.
- PRICKLE1可能具有作为结性脊柱炎早期查指标的潜力.
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