零度以下温度对大豆蛋白分离乳液的特性和结构的影响
Haiyue Hu1, Yongli Feng1, Kaiwen Zheng1
1College of Food Science and Engineering, Tianjin University of Science & Technology, Tianjin 300457, China.
Food chemistry
|September 24, 2023
概括
冷大豆蛋白分离物 (SPI) 改变了它的结构,减少了乳化. 较低的温度最初会增加表面的疏水性和二硫化键,但极端的寒冷会导致聚合,影响食品应用.
科学领域:
- 食品科学 食品科学 食品科学
- 蛋白质化学 蛋白质化学
- 材料科学 是一种材料科学.
背景情况:
- 豆蛋白分离物 (SPI) 是一种广泛使用的食品成分.
- 了解冷等加工条件如何影响SPI对于食品产品开发至关重要.
- 蛋白质结构直接影响功能性质,如乳化.
研究的目的:
- 研究不同结温度对大豆蛋白分离物 (SPI) 的结构性质的影响.
- 评估冷对SPI乳化性能的影响.
- 为优化SPI在冷食品中的使用提供见解.
主要方法:
- 豆蛋白分离物 (SPI) 的样本经过各种结温度 (-5, -20, -40,和 -80°C).
- 使用光光谱和二次结构分析 (β-sheet,β-turn,随机线圈) 分析了结构变化.
- 测量了表面的疏水性,硫和二硫化物键含量.
- 在冷处理后评估了乳化性能.
主要成果:
- 在-5°C和-20°C的冷放松了SPI结构,增加了暴露的疏水氨基酸 (Phe,Tyr,Trp).
- 表面疏水性,硫和二硫化物键表现出复杂的变化与温度下降,在-40°C和-80°C达到峰值.
- 蛋白质二次结构分析表明,在-40°C时聚合 (增加β-片含量).
- 在所有冷处理后,SPI的乳化性能显著下降.
结论:
- 结温度显著改变了SPI的结构和功能.
- 观察到的结构变化,包括在较低温度下聚合,对SPI的乳化能力产生负面影响.
- 这些发现为将SPI纳入冷食品提供了理论支持,这表明需要仔细考虑冷条件.
更多相关视频
08:59A Freeze-Thawing Method to Prepare Chitosan-Polyvinyl alcohol Hydrogels Without Crosslinking Agents and Diflunisal Release Studies
Published on: January 14, 2020
17.6K
10:20In vitro Digestion of Emulsions in a Single Droplet via Multi Subphase Exchange of Simulated Gastrointestinal Fluids
Published on: November 18, 2022
2.6K
相关概念视频
Cryo-electron Microscopy
3.4K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.4K
Protein and Protein Structure
79.7K
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...
A protein's shape is critical to its function. For example, an enzyme...
79.7K
Sublimation
771
Sublimation is the direct transformation of a solid to a gaseous state. For instance, at standard pressure and room temperature, solid carbon dioxide sublimes to gaseous carbon dioxide. The phase diagram depicts the conditions required for sublimation. This process occurs at the solid-gas phase boundary and is not observed above the triple point of the substance. The reverse of sublimation is called deposition, where a gaseous substance condenses directly into a solid. Sublimation and...
771
Physical Properties Affecting Solubility
22.7K
Solutions of Gases in Liquids
As for any solution, the solubility of a gas in a liquid is affected by the attractive intermolecular forces between solute and solvent species. Unlike solid and liquid solutes, however, there is no solute-solute intermolecular attraction to overcome when a gaseous solute dissolves in a liquid solvent since the atoms or molecules comprising a gas are far separated and experience negligible interactions. Consequently, solute-solvent interactions are the sole...
As for any solution, the solubility of a gas in a liquid is affected by the attractive intermolecular forces between solute and solvent species. Unlike solid and liquid solutes, however, there is no solute-solute intermolecular attraction to overcome when a gaseous solute dissolves in a liquid solvent since the atoms or molecules comprising a gas are far separated and experience negligible interactions. Consequently, solute-solvent interactions are the sole...
22.7K
Recrystallization: Solid–Solution Equilibria
1.1K
Recrystallization is a purification technique used to separate impurities from solid compounds. In this technique, no chemical reactions occur. Instead, it exploits physical properties only, specifically, the solubility differences between the desired compound and impurities, either at a single temperature or at different temperatures, and under other selected conditions. The solid-solution equilibrium (solubility equilibrium) of each component in the solution represents a binary phase...
1.1K
Responses to Heat and Cold Stress
13.6K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.6K
