和氧环素对移动遗传元素,抗生素耐药性基因以及土壤中的微生物群落演变的影响
Ke Li1, Ying Zhu1, Xinhua Shi1
1Advanced Materials Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250014, China.
Environmental pollution (Barking, Essex : 1987)
|September 24, 2023
概括
肥料修改土壤中的氧化环素 (OTC) 和 (Zn) 可以影响抗生素耐药性基因 (ARG) 和移动遗传元素 (MGE). 它们的联合应用可能会减少MGE和ARG,为管理土壤污染提供了洞察力.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 土壤科学 土壤科学
背景情况:
- 动物料中的抗生素和重金属通过便进入农业土壤,造成污染.
- 牛氧三环素 (OTC) 和 (Zn) 对抗生素耐药性基因 (ARG),移动遗传元素 (MGE) 和土壤微生物群落的影响尚未完全理解.
研究的目的:
- 评估OTC和Zn单独或组合对土壤环境因素,微生物,MGE和ARG的影响.
- 确定关键的微生物参与者和参与ARG动态的遗传元素.
主要方法:
- 用不同度的OTC和Zn进行受控的土壤化实验.
- 使用分子技术量化MGE和ARG的量化.
- 微生物社区分析和网络分析.
- 冗余分析以确定MGE,ARG和微生物种群之间的关系.
主要成果:
- Zn和OTC单独刺激MGE表达,但它们的组合抑制了它.
- 和OTC度的增加,以及化时间,通常会降低MGE和ARG的丰度.
- 低OTC抑制了sul基因,而高OTC抑制了tet基因.
- 坚果菌和蛋白质菌种群显著影响了ARG的丰富性.
- MGEs,特别是intI2,是ARG转移和传播的关键促进者.
结论:
- OTC 和 Zn 影响土壤中 ARG 和 MGE 的丰度和传播.
- 了解这些相互作用对于评估和减轻与污染肥料相关的生态风险至关重要.
- 结果提供了用于管理有机改土壤中的环境风险的参考值.
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