红米粉修饰 - 结合非热方法与脉冲电场 (PEF) 和使用α-氨酸酶的酶法
Raphael Lucas Jacinto Almeida1, Newton Carlos Santos1, Cecilia Elisa Sousa Muniz2
1Department of Chemical Engineering, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
International journal of biological macromolecules
|September 24, 2023
概括
使用脉冲电场 (PEF) 和α-氨酶 (AA) 进行红米粉的双重修饰提供了一种高效,环保的方法. 组合处理减少了粉的凝化,改善了糊的特性,并减少了协同作用,显示了食品应用的潜力.
科学领域:
- 食品科学与技术 食品科学与技术
- 生物材料工程 生物材料工程
- 生物物理化学 生物物理化学
背景情况:
- 本地红米粉表现出特定的形态结构,热和粘性氨基图学性质.
- 了解粉修饰对于优化食品加工和产品质量至关重要.
研究的目的:
- 通过脉冲电场 (PEF) 和α-氨酶 (AA) 来研究红米粉的双重修饰.
- 评估这些治疗对形态结构,热和粘性氨基图学特征的影响.
- 为了确定处理顺序对粉性质的影响.
主要方法:
- 红米粉用PEF (30千伏/厘米),α-氨酶 (9.0单位/毫克) 或两者的组合 (PEF+α和α+PEF) 进行处理.
- 使用了形态结构分析,X射线衍射 (XRD),FT-IR光谱,差分扫描热量计 (DSC) 和粘性氨基图.
- 评估了消化程度,颗粒形态,结晶度,双螺旋度 (DDH),秩序度 (DO),凝化温度,度,糊坚度和协同作用.
主要成果:
- PEF + α 处理显示了最高的消化率 (10.66%) 和诱导的形态变化 (延长,曲的颗粒;增加直径).
- PEF处理最大限度地降低了晶度 (21.17%),而PEF和AA都增加了DDH和降低了DO.
- 组合治疗降低了凝化温度和度,需要更少的能量;PEF + α在减少协同和逆行方面最有效.
- 处理顺序影响了结构特征,特别是当PEF在酶处理之前时.
结论:
- 用PEF和α-氨酶的双重修饰有效地改变了红米粉的特性.
- 这些处理方法减少了粉凝化的能量需求,并提高了糊的稳定性.
- 这些发现为粉修饰的环保方法提供了洞察力,并具有潜在的食品工业应用.
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