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长非编码RNA lnc-H19 对于耐力运动很重要,因为它可以维持缓慢的肌肉纤维类型

Yongqi Yue¹, Yanru Yue¹, Zeyu Fan¹

¹Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Shaanxi, China.

The Journal of biological chemistry

|September 24, 2023

概括

长非编码的H19 (lnc-H19) 保持缓慢的肌肉纤维和运动耐力. 它的缺乏促进了快速纤维特性,并损害了线粒体功能,影响了肌肉性能.

科学领域:

分子生物学分子生物学
骨肌肉生理学骨肌肉生理学
非编码RNA研究研究

背景情况:

骨肌肉的异质性源于不同的纤维类型,具有不同的代谢概况和MyHC异构体表达.
长非编码RNAs (lncRNAs) 是控制肌肉纤维类型转换的复杂分子网络中的关键调节者.

研究的目的:

研究Inc-H19在调节骨肌肉纤维类型中的作用.
为了确定Inc-H19对线粒体功能和运动表现的影响.

主要方法:

使用siRNA干扰肌肉细胞中Inc-H19表达的体外研究.
在体内研究涉及AAV-shRNA-H19在小鼠中击倒Inc-H19.
对基因和蛋白质表达 (MyHC I,MyHC IIB),线粒体形态和功能,酶活性和氧化应激标志物的分析.
通过承重游泳和四肢悬浮测试来评估运动耐力.

主要成果:

lnc-H19富含缓慢的肌肉纤维.
lnc-H19 knockdown in vitro促进了快纤维基因 (MyHC IIB) 的表达,并抑制了慢纤维基因 (MyHC I) 的表达.
在体内,Inc-H19缺乏导致慢MyHC表达减少,线粒体损伤,氧化代谢降低,抗氧化能力受损.
受Inc-H19敲击的小鼠表现出减弱的运动耐力.

结论:

lnc-H19在维持缓慢的肌肉纤维类型中起着至关重要的作用.
lnc-H19对于维护线粒体功能和骨肌肉中抗氧化能力至关重要.
lnc-H19有助于维持运动耐力,突出其作为改善肌肉功能的治疗点的潜力.

关键词:

耐力运动是一种耐力运动.在H19中,H19是H1N1的.线粒体中的线粒体.肌纤维类型肌纤维类型

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