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非小细胞肺癌表观基因组在吸烟者和从未吸烟者中表现出改变的DNA甲基化

Jennifer A Karlow¹, Erica C Pehrsson¹, Xiaoyun Xing¹

¹Department of Genetics, Washington University School of Medicine, St. Louis, MO 63110, USA; The Edison Family Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St. Louis, MO 63110, USA.

Genomics, proteomics & bioinformatics

|September 24, 2023

概括

在非小细胞肺癌 (NSCLC) 中,DNA甲基化的表观遗传变化很常见. 这些变化,特别是从不吸烟者的低甲基化,影响瘤表型和基因调节.

科学领域:

在瘤学瘤学.
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
基因组学就是基因组学.

背景情况:

表观遗传改变,包括DNA甲基化,对于癌症的发展和进展至关重要.
了解非小细胞肺癌 (NSCLC) 的这些变化对于确定治疗点至关重要.

研究的目的:

为了研究NSCLC中全基因组DNA甲基化变化的研究.
为了比较吸烟者和从未吸烟者之间的甲基化概况.
将甲基化变化与瘤表型和基因表达相关联.

主要方法:

使用甲基化DNA免疫沉测序 (MeDIP-seq) 和甲基化敏感限制酶测序 (MRE-seq) 的全基因组DNA甲基化概况.
分析了17个初级NSCLC瘤和10个匹配的正常肺样本.
基于吸烟状态的甲基化模式的比较.

主要成果:

在像FAM83A,SEPT9,PCDH7,NKX2-1和SOX17这样的基因中确定了反复的促进子甲基化变化.
观察到吸烟状态特定的甲基化变化,从未吸烟者显示更多的低甲基化区域.
检测到促进体外广泛的甲基化变化,包括重复元素,以及在低甲基化区域中转录因子结合基因的丰富.

结论:

DNA甲基化变化对NSCLC表型有显著的贡献.
吸烟者和非吸烟者之间的甲基化模式不同,这表明有不同的病因路径.
甲基化变化可能会影响活性染色体状态内的基因调节,影响细胞类型特定的表达程序.

关键词:

通过DNA甲基化.表观基因组学是指表观基因组学.这就是M&M的意义.非小细胞肺癌是非小细胞肺癌.甲基CRFF是一种

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