来自Anthriscus sylvestris (L.) Hoffm根的二烯胺和二烯胺
Yan-Ling Liu1, Yan-Gang Cao1, Ying Niu1
1School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, China; The Engineering and Technology Center for Chinese Medicine Development of Henan Province China, Zhengzhou, 450046, China.
Phytochemistry
|September 24, 2023
概括
来自Anthriscus sylvestris根的新化合物显示出抑制肺动脉高血压的潜力. 这些天然产品调节细胞增殖,细胞亡和光滑肌肉细胞中的炎症因素.
科学领域:
- 自然产品化学 自然产品化学
- 药理学 药理学是指药理学的学科.
- 分子生物学分子生物学
背景情况:
- 肺动脉高血压 (PAH) 涉及肺动脉光滑肌细胞 (PASMCs) 的异常增殖.
- 仙人掌 (Anthriscus sylvestris) 是一种具有潜在药用功能的植物来源.
- 鉴定PAH的新型治疗剂至关重要.
研究的目的:
- 从Anthriscus sylvestris根源中分离和描述新的化合物.
- 评估这些化合物对缺氧诱导的PASMC增殖的抑制作用.
- 调查它们的抗增殖活性背后的分子机制.
主要方法:
- 使用NMR光谱学,HRESIMS和电子圆形二元化对化合物的隔离和结构阐明.
- MTT测定用于评估细胞活力和增殖.
- 定量实时PCR测量与亡相关的基因 (Bcl-2,BAX,Caspase3) 和炎症因子 (IL-6) 的mRNA表达.
主要成果:
- 从Anthriscus sylvestris中鉴定出了8种新的化合物,包括二烯类和烯类.
- 一些孤立的化合物,特别是 (-) - 1, (+) - 1, (-) - 2, (+) - 3,4,8 - 10,14和15,显示出显著抑制PASMC的扩散.
- 这些化合物调节了关键的亡和炎症标志物的表达.
结论:
- 草 (Anthriscus sylvestris) 是一种新型生物活性化合物的来源,在PAH中具有潜在的治疗应用.
- 已识别的化合物通过调节 PASMC 中的亡和炎症途径来发挥其作用.
- 对这些化合物的进一步研究可能会导致肺动脉高血压的新疗法.
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