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在患有胃肠道癌症的患者中进行小型非编码RNA分析

Alessio Molfino¹, Marc Beltrà^2,3, Maria Ida Amabile⁴

¹Department of Translational and Precision Medicine, Sapienza University of Rome, Rome, Italy.

Journal of cachexia, sarcopenia and muscle

|September 24, 2023

概括

小型非编码RNAs (sncRNAs) 在胃肠道癌症患者中失调,影响肌肉消耗. 在癌症患者中,循环中的microRNA与肌肉度相关,而不是体重减轻.

科学领域:

分子生物学分子生物学
遗传学遗传学是一个
在瘤学瘤学.

背景情况:

小型非编码RNAs (sncRNAs),包括microRNAs和PIWI相互作用RNAs (piRNAs),调节基因表达.
肌肉特定的microRNAs (myomiRs) 与癌症引起的肌肉衰竭有关.
胃肠道癌症患者表现出改变的基因表达模式.

研究的目的:

在胃肠道癌症患者的骨肌肉和血中分析sncRNA.
研究差异表达的sncRNA与肌肉水平之间的关联.
了解sncRNAs在与癌症相关的肌肉衰竭中的作用.

主要方法:

招募了患有胃肠道癌症或良性疾病的外科患者.
采集了血液样本和直腹肌肌肉活检.
使用RNAseq (肌肉) 和qPCR (血) 评估的sncRNA.
通过基于CT扫描的骨肌肉指数 (SMI) 确定肌肉度.

主要成果:

与对照组相比,癌症患者表现出循环中的microRNAs (miR-21-5p,miR-133a-3p,miR-15b-5p) 的不同表达.
肌肉sncRNA分析显示,癌症患者的microRNAs和piRNAs显著失调.
在中度至高肌肉强度的男性中观察到miR-133a-3p和miR-206的增加.
下调的微RNAs (miR-15b-5p,miR-106a-5p,miR-106b-5p) 针对涉及到无素连接酶/转移酶活动的基因.

结论:

肌肉微RNA和piRNA在癌症患者中失调,微RNA的性别特异性模式.
循环中的microRNAs的变化与肌肉度的程度有关,而不是体重减轻.
这些发现突显了sncRNAs在与癌症相关的肌肉病理生理学中的作用.

关键词:

生物标志物生物标志物胃肠道癌症是什么意思肌肉性肌肉性肌肉性骨架肌肉是一个骨肌肉.小型非编码RNAs的编码.这就是miRNAs.这就是piRNAs.

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