相关实验视频

Updated: Jul 15, 2025

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Published on: December 12, 2019

一个保存的HOTAIRM1-HOXA1调节轴为神经元分化做出早期贡献

Dana Segal¹, Samy Coulombe^1,2, Jasper Sim¹

¹Department of Biochemistry, and Rosalind & Morris Goodman Cancer Institute, McGill University, Montréal, Québec, Canada.

|September 25, 2023

概括

长非编码RNAHOTAIRM1对神经元分化至关重要,与HOXA1合作,在早期发育过程中调节多能性基因. 这种保存的HOTAIRM1-HOXA1轴指导从干细胞到神经元的过渡.

科学领域:

* 分子生物学 * 分子生物学
* 发育生物学发育生物学
* 基因组学是一个学科.

背景情况:

* 长非编码RNAs (lncRNAs) 越来越多地被认为是它们在基因调节中的作用.
*HOTAIRM1显示了哺乳动物的高序列保护,表明关键功能.
* 之前的工作确立了HOTAIRM1在通过表观遗传修饰调节HOXA基因表达中的作用.

研究的目的:

* 研究HOTAIRM1在神经元分化中的作用.
* 为了阐明HOTAIRM1和HOXA1转录因子之间的相互作用.
* 了解HOTAIRM1如何在细胞命运过渡期间影响核心多能网络.

主要方法:

*基于细胞的测试用于研究神经元分化.
*对基因表达模式的分析,包括多能性因子.
*研究蛋白质-RNA相互作用和染色质修饰.

主要成果:

*HOTAIRM1对于早期神经元分化的正确进展至关重要.
*HOTAIRM1与HOXA1转录因子结合,调节其下游目标.
*HOTAIRM1影响NANOG/POU5F1/SOX2多能网络,影响过渡到差异化状态.
* HOXA1 副本的耗尽 HOTAIRM1 对多能性因子的影响.

结论:

* HOTAIRM1 作为一个关键的调节器,在从多能到分化神经元状态的过渡中起作用.
*一个保存的HOTAIRM1-HOXA1核糖蛋白复合体形成了对神经元发育至关重要的调节轴.
*HOTAIRM1通过与HOXA1.1的相互作用来调节核心多能网络.

关键词:

这里是HOTAIRM1的房间.在HOXA1中,HOXA1是指HOXA1.这是一个LncRNARNA.这是一个NANOGNANOG.这就是SOX2的含量.在这种情况下,染色染色素不同化的差异化差异化.表观遗传学是指表观遗传学.神经发生神经发生.多能性的多能性.

更多相关视频

Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation

Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation

Published on: February 28, 2021

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo

Published on: February 2, 2016

相关概念视频

Master Transcription Regulators

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

Hedgehog Signaling Pathway

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...

Determination

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...

Regulation of Expression Occurs at Multiple Steps

Regulation of Expression Occurs at Multiple Steps

Zygotic Development And Stem Cell Formation

Zygotic Development And Stem Cell Formation

The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...

Gastrulation

Gastrulation

Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...