非酶性葡萄糖检测采用氧化/奇托桑改性丝印电极,嵌入到注入流量分析系统中
Wesley J McCormick1, Eva McLoughlin1, Denis McCrudden1
1Department of Life and Physical Sciences, Atlantic Technology University, Letterkenny, Co. Donegal, F92 FC93, Ireland. wesley.mccormick@atu.ie.
Analytical methods : advancing methods and applications
|September 25, 2023
概括
一个新的氧化和酸盐改性电极为流注分析提供了敏感,选择性和可重复的非酶性葡萄糖检测. 这一进步在真实血清样本中提供了准确的葡萄糖量化.
科学领域:
- 电化学 电化学 电化学
- 纳米材料科学 科学 纳米材料科学
- 分析化学 分析化学
背景情况:
- 精确的葡萄糖监测对于糖尿病管理至关重要.
- 开发敏感和选择性的电化学传感器是一个持续的挑战.
- 非酶检测方法在稳定性和成本方面具有优势.
研究的目的:
- 开发一种新型的印碳电极,该碳电极经过氧化纳米粒子和奇托 (Ni(OH) 2/CS/SPCE) 的修改.
- 为了研究其对非酶体流量注入葡萄糖的强度计检测的有效性.
- 在真实的人类血清样本中验证其性能.
主要方法:
- 电极修改通过滴一个Ni (OH) 2/CS纳米复合材料到一个印碳电极.
- 使用循环电压计 (CV) 进行电化学表征.
- 在流量注入分析 (FIA) 中的优化和应用,用于葡萄糖检测.
主要成果:
- (OH) 2/CS/SPCE在0.55V时表现出氧化葡萄糖的催化活性.
- 最佳的FIA条件被确定为0.60V和1.5mLmin-1流速.
- 实现了广泛的线性检测范围 (0.210.0 mM),具有高灵敏度 (913 μA mM-1 cm-2) 和低检测极限 (0.0174 mM).
- 证明了出色的重复性 (RSD = 0.47%) 和可重复性 (RSD = 2.52%).
- 在常见的血液干扰中显示出对葡萄糖的高选择性.
- 在真实血清样本中得到验证,结果与医院方法相似.
结论:
- 开发的Ni(OH) 2/CS/SPCE是一个有前途的平台,用于敏感和选择性的非酶性葡萄糖检测.
- 流量注入电压计系统为葡萄糖量化提供了强大的和实用的方法.
- 这种方法有可能用于临床诊断和生物传感应用.
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