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Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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相关实验视频

Updated: Jul 15, 2025

A Microfluidic Technique to Probe Cell Deformability

A Microfluidic Technique to Probe Cell Deformability

Published on: September 3, 2014

最近在变形辅助微流体细胞分类技术的进展.

Jingjing Sun¹, Xiwei Huang¹, Jin Chen¹

¹Ministry of Education Key Lab of RF Circuits and Systems, Hangzhou Dianzi University, China. huangxiwei@hdu.edu.cn.

|September 25, 2023

概括

此摘要是机器生成的。

变形辅助微流体细胞分类提供了一个有希望的无标签方法,以克服基于尺寸的方法的局限性. 这项技术利用细胞变形性来改善生命科学和临床研究中的细胞分析.

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Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

相关实验视频

Last Updated: Jul 15, 2025

A Microfluidic Technique to Probe Cell Deformability

A Microfluidic Technique to Probe Cell Deformability

Published on: September 3, 2014

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

科学领域:

生物物理学的生物物理.
微流体学微流体学
细胞生物学细胞生物学

背景情况:

细胞分类对于生命科学和临床研究至关重要.
无标签,以大小为基础的微流体分类面临由于细胞大小异质性和重叠的挑战.
细胞变形性提供了一个额外的生物物理标记,用于更准确的细胞分类.

研究的目的:

审查最近在变形辅助微流体细胞分类技术的进展.
根据可变形性特征和力应用,对这些技术进行分类.
讨论性能,应用,挑战和未来前景.

主要方法:

分类为间接 (运输时间,基于图像) 和直接 (微观结构,基于水力动力学) 的方法.
分析细胞变形性是如何表征的,以及力量是如何施加的.
对每个方法的分离性能和应用场景的审查.

主要成果:

变形辅助方法提供了一个额外的维度超出细胞大小进行分类.
间接方法使用过渡时间或图像分析.
直接方法使用微观结构或水力动力学力来评估可变性.

结论:

变形辅助的微流体细胞分类增强了无标签的细胞分析.
这些技术解决了纯粹基于大小的分类方法的局限性.
未来的开发具有在细胞研究和诊断领域更广泛应用的巨大潜力.