一种用于控制多药耐药的禽类病原菌大肠杆菌 (APEC) 感染的菌体
Lan Yao1,2, Yinli Bao3, Jiangang Hu1
1Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Frontiers in cellular and infection microbiology
|September 25, 2023
概括
像PEC9这样的菌体 (菌体) 对抗多药耐药的禽类致病菌大肠杆菌 (APEC) 有希望. 本研究详细介绍了PEC9的情况.
科学领域:
- 微生物学 微生物学
- 病毒学 病毒学
- 基因组学就是基因组学.
背景情况:
- 抗生素耐药性是一个日益严重的全球健康问题,需要替代抗菌战略.
- 菌体 (菌体) 是感染细菌的病毒,正在被探索作为抗生素的潜在替代品.
- 禽病原性大肠杆菌 (APEC) 在家禽行业造成重大经济损失,并构成动物感染风险.
研究的目的:
- 隔离和表征一种对禽病原菌大肠杆菌 (APEC) 有效的性菌.
- 评估隔离的菌体的基因组特征和稳定性.
- 在小鼠模型中评估菌体对APEC感染的治疗疗效.
主要方法:
- 从养殖场便中分离和净化菌体PEC9.
- 电子显微镜用于形态学,一阶段生长曲线,稳定性测试 (pH,温度) 和体外抗菌/生物膜抑制测试.
- 全基因组测序,生物信息学分析和家族遗传树的构建.
- 在小鼠模型中进行的体内疗效研究与APEC有挑战.
主要成果:
- 体PEC9,一种类似于细菌病毒的粒子,有效地溶解了两种大肠杆菌血清型 (O1和O2).
- 最佳感染多重性 (MOI) 为0.1,潜伏期为20分钟,爆发大小为68个PFU/细胞.
- 在pH 3-11和高达50°C的温度下,PEC9表现出广泛的稳定性,抑制浮游生物的生长和生物膜的形成.
- 基因组测序揭示了44,379 bp dsDNA基因组与56个ORF,没有检测到毒素或毒性基因.
- 菌体PEC9显著改善了APEC感染小鼠的生存率,减少了细菌负载,并减轻了器官病变.
结论:
- 菌体PEC9是一种强大的菌体,具有良好的基因组特征和广泛的稳定性.
- PEC9在体外和体内对APEC具有显著的疗效,包括生物膜抑制.
- 菌体PEC9代表了开发菌体治疗APEC感染的有希望的候选人,作为抗生素的替代品.
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