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Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test
Published on: April 9, 2012
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在检测肺结核的舌头扫描的性能
Christopher S Ealand1, Astika Sewcharran1, Julian S Peters1
1Department of Science and Innovation/National Research Foundation Centre of Excellence for Biomedical TB Research, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand and the National Health Laboratory Service, Johannesburg, South Africa.
Frontiers in cellular and infection microbiology
|September 25, 2023
概括
舌头扫描显示结核病 (TB) 检测率低于唾液,但生长补充剂可能会改善诊断,特别是在HIV阳性个体中. 需要进一步的研究,以在更大的研究中证实这些发现.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 诊断技术 诊断技术 诊断技术
背景情况:
- 口腔和舌头扫描可以检测肺结核 (TB) 的成年人中的Mycobacterium结核病 (Mtb).
- 不同培养结核菌 (DCTB) 需要特定的生长因子 (培养过物 - CF) 来检测.
- 目前的分子分析显示出有希望的结果,但基于培养的检测需要改进.
研究的目的:
- 为了比较唾液和舌头拭子的诊断性能,以检测结核病.
- 评估DCTB测试的实用性,有或没有CF补充,以改善结核病诊断.
- 评估艾滋病毒状况对诊断产量的影响.
主要方法:
- 从89名参与者那里收集了唾液和舌头拭子.
- 样本使用菌根生长指标管 (MGIT) 试验,奥拉胺涂抹,GeneXpert和DCTB试验 (有/没有CF) 进行了测试.
- 还评估了一种替代的涂抹染料 (DMN-Trehalose).
主要成果:
- 唾液样本在所有测试方法中显示出较高的阳性率,而不是舌头拭子.
- 用CF增强的DCTB测定为唾液和舌头拭子的阳性率更高.
- 从艾滋病毒阳性参与者的舌头拭子中,DCTB阳性显著增加.
- 在DMN-Trehalose涂抹染料上,诊断产量没有显著提高.
结论:
- 与唾液相比,舌头拭片在结核病诊断测试中产生较低的细菌负载.
- 用CF补充生长剂可以通过DCTB测定提高结核病检测,特别是在HIV阳性个体.
- 需要进行更大规模的研究来验证这些发现,并探索舌头拭子用增强培养方法的潜力.
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